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Preparations of ginsenoside Rg1 immunogen and125I-labeled antiğen

  • Radiopharmaceuticals, Labelled Compounds and RIA
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Abstract

This work began with the side chain of ginsenoside Rg1 which was acetylized with Ac2O-Pyr and oxidized by OsO4 and NaIO4 to give ginsenoside Rg1 aldehyde(2), which was further converted into an unsaturated ester(3) by Wittig-Horner reaction. The unsaturated ester(3) was treated with N2H4 and HNO2 to yield Rg1 azide(4) which was directly conjugated with BSA to give immunogen: ginsenoside Rg1-BSA conjugate(5). This azide(4) was conjugated with tyramine to give ginsenoside Rg1-tyramine conjugate which was labelled with free Na125I by CH-T to yield125I-labeled antigen: gensenoside Rg1-125I-tyramine(6). The labelling rate was 40%–50% and specific activity was 3.0–4.0 MBq/μg.

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Authors and Affiliations

  1. China Institute of Atomic Energy, P.O. Box 275-39, 1-2413, Beijing, P.R. China

    Mei Congbiao, Wang Qiang, Yi Mingguang & Xia Zuping

Authors
  1. Mei Congbiao
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  2. Wang Qiang
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  3. Yi Mingguang
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  4. Xia Zuping
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Congbiao, M., Qiang, W., Mingguang, Y. et al. Preparations of ginsenoside Rg1 immunogen and125I-labeled antiğen. Journal of Radioanalytical and Nuclear Chemistry, Articles 206, 201–203 (1996). https://doi.org/10.1007/BF02039644

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  • DOI: https://doi.org/10.1007/BF02039644

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