Abstract
Silanized magnetic particles have been developed by means of a number of different methods for coupling antibodies for use in radioimmunoassay (RIA) and immunoradiometric assay (IRMA) of thyroid related hormones. Carbodiimide, glutaraldehyde, glutaric anhydride and spacer-arms coupling methods were studied for immobilization of triiodothyronine (Ts), thyroxine (T4) and thyroid stimulating hormone (TSH) antibodies on magnetic particles respectively. The effect of the magnetic particle preparation on the couplings was discussed. The influence of coupling conditions on the preparation of magnetic particle antibodies and the assay performance was also investigated. The storage stability and reproducibility of the magnetic particle T3, T4 and TSH antibodies were good enough for use in total T3, total T4 RIA and TSH IRMA. A comparison of five types of magnetic particles from different sources for coupling TSH antibody for TSH IRMA application was made. Silanized magnetic particles and other types of particles showed comparable results. Five TSH antibodies, including two polyclonal and three monoclonal antibodies coupled to silanized magnetic particles respectively, were used in TSH IRMA. It was found that each of them and a commercial monoclonal TSH antibody as label formed good sandwich partners and gave a high signal to low levels of TSH.
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This work was performed in the scope of the Coordinated Research Programme “Antibodies Immobilized on Magnetic Particles for Radioimmunoassay and Immunoradiometric Assay. Hormones” (Contract No. 6486/RB) organized and supported by the International Atomic Energy Agency, Vienna. The authors express their thanks to the Agency for supporting this research.
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Decun, S., Jiaxiu, S., Sha, L. et al. Preparation of magnetic particle antibodies for radioimmunoassay and immunoradiometric assay of thyroid related hormones. Journal of Radioanalytical and Nuclear Chemistry, Articles 206, 189–200 (1996). https://doi.org/10.1007/BF02039643
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DOI: https://doi.org/10.1007/BF02039643