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Evaluation of methods for the separation of trimethylselenonium ion as a major, minor or general metabolite using neutron activation analysis

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Abstract

Trinethylselenonium (TMSe) ion was separated by dual-column ion-exchange chromatography and assayed by neutron activation analysis. The TMSe content of the selenium in human urine was found to be (14±2)%, consistent with literature values. An altemate, published, multi-step procedure employing Reineckate precipitation followed by decomposition/volatilization conversion of selenium into dimethylselenide, perchloric acid digestion into selenite and subsequent analysis employing inductively coupled plasma mass spectrometry (ICP-MS) was evaluated. A maximum recovery of TMSe was estimated at 32%. It has been suggested that losses in each step of a multi-step procedure yield low recoveries as reported in the literature.

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Authors and Affiliations

  1. Department of Chemistry, University of Nebraska-Lincoln, 68588-0304, Lincoln, Nebraska, (USA)

    E. P. Rack & D. M. Shoop

  2. Medical Research, Department of Veterans Affairs Medical Center, 68105, Omaha, Nebraska, (USA)

    A. J. Blotcky & J. P. Claassen

Authors
  1. A. J. Blotcky
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  2. J. P. Claassen
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  3. E. P. Rack
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  4. D. M. Shoop
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Blotcky, A.J., Claassen, J.P., Rack, E.P. et al. Evaluation of methods for the separation of trimethylselenonium ion as a major, minor or general metabolite using neutron activation analysis. Journal of Radioanalytical and Nuclear Chemistry, Articles 181, 395–400 (1994). https://doi.org/10.1007/BF02037645

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  • DOI: https://doi.org/10.1007/BF02037645

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