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A new method for the production of a magnetic immunosorbent used in radioimmunoassay and immunoradiometric assay

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Abstract

A method has been developed enabling the direct coupling of first or second antibody to finely dispersed magnetite (Fe3O4). The immunosorbent thus produced was applied in various radioimmunoassay systems (T3, T4, TSH, Cortisol) for the separation of bound and free antigens. The elimination of the need for “precoating” the magnetic particles with a polymer has several advantages. One of them lies in the ease of one-step production of the immunosorbent and other is the high antibody/magnetite ratio. The influence of the concentration of the immunosorbent and detergent (TWEEN 20 or TRITON X-100) on the assay parameters (Bo, NSB, etc.) has been systematically investigated and the optimum concentration of the magnetizable particles and detergent has been determined. The reliability of magnetic separation has been validated by comparing it with the conventional PEG separation method.

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Authors and Affiliations

  1. Institute of Isotopes Co., Ltd, P.O. Box 77, 1525, Budapest, (Hungary)

    G. Tóth, V. Keszei & I. Sárándi

Authors
  1. G. Tóth
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  2. V. Keszei
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  3. I. Sárándi
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Additional information

This work was performed in the scope of the Coordinated Research ProgrammeAntibodies Immobilized on Magnetic Particles for Radioimmunoassay and Immunoradiometric Assay (No6 487CF) organized and supported by the International Atomic Energy Agency, Vienna. The authors express their thanks to the Agency for supporting this work.

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Tóth, G., Keszei, V. & Sárándi, I. A new method for the production of a magnetic immunosorbent used in radioimmunoassay and immunoradiometric assay. Journal of Radioanalytical and Nuclear Chemistry, Articles 181, 263–279 (1994). https://doi.org/10.1007/BF02037633

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  • DOI: https://doi.org/10.1007/BF02037633

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