Skip to main content
SpringerLink
Log in

An external standard method for quantification of human cytomegalovirus by PCR

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

An external standard method for PCR quantification of HCMV was reported. [α-32P]dATP was used as a tracer.32P-labelled specific amplification product was separated by agarose gel electrophoresis. A gel piece containing the specific product band was excised and counted in a plastic scintillation counter. Distribution of [α-32P]dATP in the electrophoretic gel plate and effect of separation between the32P-labelled specific product and free [α-32P]dATP were observed. A standard curve for quantification of HCMV by PCR was established and detective results of quality control templets were presented. The external standard method and the electrophoresis separation effect were appraised. The results showed that the method could be used for relative quantification of HCMV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. K. Saiki, S. Scharf, F. Faioona, K. B. Mullis, G. T. Horn, H. A. Erlich, N. Arnheim, Science, 230 (1985) 1350.

    PubMed  Google Scholar 

  2. Shen Rongsen, Ma Liren, Huang Haiyan, Liu Yaoqing, Xing Ruiyun, Yang Hong, Bull. Acad. Milit. Med. Sci., 19 (1995) 204 (Chinese).

    Google Scholar 

  3. S. Diviacco, P. Norio, L. Zentilin, S. Menzo, M. Clementi, G. Biamonti, S. Riva, A. Falaschi, M. Giacca, Gene, 122 (1992) 313.

    PubMed  Google Scholar 

  4. D. Zipeto, F. Baldanti, D. Zella, M. Furione, A. Cavicchini, G. Milanesi, G. Gerna, J. Virol. Methods, 44 (1993) 45.

    PubMed  Google Scholar 

  5. H. E. Chehadeh, G. Zerlauth, J. W. Mannhalter, Biotechniques, 18 (1995) 26, 28.

    Google Scholar 

  6. R. Thiery, P. Boutin, C. Arnauld, A. Jestin, Biotechniques, 18 (1995) 212.

    Google Scholar 

  7. M. Becker-Andre, K. Hahlbrock, Nucleic Acids Res., 17 (1989) 9437.

    PubMed  Google Scholar 

  8. G. Gilliland, S. Perrin, K. Blanchard, F. Bunn, Proc. Natl. Acad. Sci., USA, 87 (1990) 2725.

    Google Scholar 

  9. J. C. Fox, P. D. Griffiths, V. C. Emery, J. Gen. Virol., 73 (1992) 2405.

    PubMed  Google Scholar 

  10. K. Henco, M. Heibey, Nucleic Acids Res., 18 (1990) 6733.

    PubMed  Google Scholar 

  11. R. A. Frye, C. C. Benz, E. Liu, Oncogene, 4 (1989) 1153.

    Google Scholar 

  12. D. E. Kellogg, J. J. Sninsky, S. Kwok, Anal. Biochem., 189 (1990) 202.

    PubMed  Google Scholar 

  13. A. M. Vlieger, A. M. J. C. Medenblik, R. P. M. V. Gijlswijk, H. J. Tanke, M. V. D. Ploeg, J. W. Gratama, A. K. Raap, Analyt. Biochem., 205 (1992) 1.

    PubMed  Google Scholar 

  14. Y. E. Khudyakov, L. Gaur, J. Singh, P. Patel, H. A. Fields, Nucleic Acids Res., 22 (1994) 1320.

    PubMed  Google Scholar 

  15. R. J. Wiesner, B. Beinbrech, J. C. Ruegg, Nature, 366 (1993) 416.

    PubMed  Google Scholar 

  16. N. C. P. Cross, Br. J. Haematol., 89 (1995) 693.

    PubMed  Google Scholar 

  17. U. Reischl, B. Kochanowski, Mol. Biotechnol., 3 (1995) 55.

    PubMed  Google Scholar 

  18. L. Raeymaekers, Anal. Biochem., 214 (1993) 582.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Radiation Medicine, 27 Taiping Road, 100850, Beijing, P. R. China

    Shen Rongsen, Ma Liren, Zhou Fengqi & Luo Qingliang

Authors
  1. Shen Rongsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ma Liren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhou Fengqi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Luo Qingliang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rongsen, S., Liren, M., Fengqi, Z. et al. An external standard method for quantification of human cytomegalovirus by PCR. J Radioanal Nucl Chem 221, 143–147 (1997). https://doi.org/10.1007/BF02035256

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02035256

Keywords

Search

Navigation