Hepatitis C virus loading was evaluated using modifications of PCR product detection: terminal point fluorescent detection, real time assay, and agarose gel electrophoresis. The advantages and flaws of the real time PCR and electrophoretic detection of amplification products for evaluation of the quantity of virus particle copies are evaluated.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 8, pp. 232-235, August, 2015
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Pogosyan, G.P., Konovalova, A.A., Protas, V.V. et al. Efficiency of Measurements of Hepatitis C Virus RNA. Bull Exp Biol Med 160, 264–266 (2015). https://doi.org/10.1007/s10517-015-3145-9
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DOI: https://doi.org/10.1007/s10517-015-3145-9