Skip to main content
SpringerLink
Log in

Neutralization of the oedematogenic activity of Bothrops Jararaca venom on the mouse paw by an antibothropic fraction isolated from Opossum (Didelphis Marsupialis) serum

  • Inflammation and Immunomodulation
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

The pharmacological modulation of mice paw oedema produced byBothrops jararaca venom (BJV) has been studied. Intraplantar injection of BJV (1–30 μg/paw) produced a dose-and time-related oedema, which was maximal 30 min after injection, reduced gradually thereafter and disappeared over 48h. BJV heated at 100°C for 5 or 15 min blocked local hemorrhage and caused partial inhibition of its oedematogenic activity. The BJV oedema was not inhibited by the anti-histamine meclizine, the inhibitor of histamine and serotonin, cyproheptadine, PAF-acether antagonist WEB 2170 or by the anti-leukotrienes C4/D4, LY 171883. Dexamethasone, aspirin, indomethacin, and the dual cyclooxygenase and lipoxygenase inhibitor BW 755C inhibited BJV-induced oedema indicating that arachidonic acid metabolism products via the cyclooxygenase pathway participate in its genesis and/or maintenance. The antibothropic fraction (ABF) (25–200 μg/paw) isolated fromDidelphis marsupialis serum neutralized the oedema induced by the venom with and without heating, the hemorrhage induced by BJV and partially blocked the oedema induced by bradykinin and by cellulose sulphate. The oedema produced by histamine, serotonin, PAF-acether or leukotriene C4 was not inhibited.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. A. Minton Jr.,Snakes and snake venoms. InVenom Diseases, pp. 107–108, Thomas Springfield 1974.

  2. G. G. Habermehl,Venomous animals and their toxins. InReptilia (Reptiles), pp. 130–180, Springer, Berlin 1981.

    Google Scholar 

  3. J. M. Gutiérrez, F. Chaves, R. Bolaños, L. Cerdas, E. Rojas, O. Arroyo and E. Portilla,Neutralización de los efectos locales del veneno de Bothrops asper por un antiveneno polivalente. Toxicon19, 493–500 (1981).

    PubMed  Google Scholar 

  4. O. Vital Brazil,Peçonhas. InFarmacodinâmica. (Ed. C. E. Corbert), pp. 1044–1074, Guanabara Koogan, Rio de Janeiro 1982.

    Google Scholar 

  5. H. F. Deutsh and C. R. Diniz,Some proteolytic activities of snake venoms. J. Biol. Chem.216, 17–26 (1955).

    PubMed  Google Scholar 

  6. J. M. Jiménez-Porras,Biochemistry of snake venoms. Clin. Toxicol.3, 389–431 (1970).

    PubMed  Google Scholar 

  7. S. Iwanaga and T. Suzuki,Enzymes in snake venoms. InSnake Venoms, Handbook of Experimental Pharmacology, vol. 52 (Ed. C. Y. Lee) pp. 61–158, Springer, Berlin 1979.

    Google Scholar 

  8. B. B. Vargaftig, N. Bhargava and I. L. Bonta,Haemorrhagic and permeability increasing effects of “Bothrops jararaca” and other Crotalidae venoms as related to amine or kinin release. Agents and Actions4, 163–168 (1974).

    PubMed  Google Scholar 

  9. A. Ohsaka,Hemorrhagic, necrotizing and oedema forming effects of snake venom. InSnake Venoms. Handbook of Experimental Pharmacology, vol. 52. (Ed. C. Y. Lee) pp. 480–546. Springer, Berlin 1979.

    Google Scholar 

  10. B. J. Hawgood,Physiological and pharmacological effects of rattlesnake venoms. InRattlesnake Venoms. Their Actions and Treatment. (Ed. T. Tu Anthony) pp. 121–162, Marcel Dekker, New York 1982.

    Google Scholar 

  11. B. Lomonte,Edema-forming activity of Bushmaster (Lachesis muta stenophrys) and Central American rattlesnake (Crotalus durissus durissus) venoms and neutralization by a polyvalent antivenom. Toxicon23, 173–176 (1985).

    PubMed  Google Scholar 

  12. H. A. Trebien and J. B. Calixto,Pharmacological evaluation of rat paw oedema induced by Bothrops jararaca venom. Agents and Actions26, 292–300 (1989).

    PubMed  Google Scholar 

  13. J. Vellard,Resistencia de los “Didelphis” (Zarigueya) a los venenos ofidicos. Rev. Brasil. Biol.5, 463–467 (1945).

    Google Scholar 

  14. M. Ovadia, B. Moav and E. Kochva,Factors in the blood serum of Vipera palaestinae neutralizing fractions of its own venom. Toxicon13, 113 (1975).

    Google Scholar 

  15. M. Ovadia and E. Kochva,Neutralization of Viperidae and Elapidae snake venoms by sera of different animals. Toxicon15, 541–547 (1977).

    PubMed  Google Scholar 

  16. H. Moussatché, A. Yates, F. Leonardi and L. Borche,Experimentos sobre la resistencia del Didelphis venezolano (rabi-pelado) a los venenos de serpientes. Acta Cient. Venezolana29, 55 (1978).

    Google Scholar 

  17. J. C. Perez, W. C. Haws, V. E. Garcia and B. M. Jennings,Resistance of warm-blooded animals to snake venoms. Toxicon16, 375–383 (1978a).

    PubMed  Google Scholar 

  18. J. C. Perez, W. C. Haws and C. H. Hatch,Resistance of woodrats (Neotoma micropus) to Crotalus atrox venom. Toxicon16, 198–200 (1978b).

    PubMed  Google Scholar 

  19. H. Moussatché and F. Leonardi,Estudios de protección con sueros de mamiferos y reptiles a los venenos de serpientes Crotalidae. Acta Cient. Venezolana33, 151 (1981).

    Google Scholar 

  20. G. B. Domont, J. Perales and H. Moussatché,Natural antisnake venom proteins. Toxicon29, 1183–1194 (1991).

    PubMed  Google Scholar 

  21. H. Moussatché, A. Yates, F. Leonardi and L. Borche,Mechanisms of resistance of the opossum to some snake venoms. Toxicon17, 130 (1979).

    Google Scholar 

  22. J. Perales, R. Muñoz and H. Moussatché,Isolation and partial characterization of a protein fraction from the opossum (Didelphis marsupialis) serum, with protecting property against the Bothrops jararaca snake venom. Ann. Acad. brasil. Ciênc.58, 155–162 (1986).

    Google Scholar 

  23. J. Perales, R. Muñoz, S. Graterol, O. Oviedo and H. Moussatché,New findings on the purification and characterization of an anti-bothropic factor from Didelphis marsupialis (opossum) serum. Brazilian J. Med. Biol. Res.22, 25–28 (1989).

    Google Scholar 

  24. H. Moussatché and J. Perales,Factors underlying the natural resistance of animals against snake venoms. Mem. Ins. Oswaldo Cruz84, 391–394 (1989).

    Google Scholar 

  25. S. H. Ferreira,A new method for measuring variations of rat paw volume. J. Pharmacol.31, 648 (1979).

    Google Scholar 

  26. J. M. Gutiérrez, O. Arroyo and R. Bolaños,Mionecrosis, hemorragia y edema inducidos por el veneno de Bothrops asper en ratón blanco. Toxicon18, 603–610 (1980).

    PubMed  Google Scholar 

  27. L. S. Queiroz, H. Santo Neto, M. T. Assakura, A. P. Reichl and F. R. Mandelbaum,Pathological changes in muscle caused by haemorrhagic and proteolytic factors from Bothrops jararaca snake venom. Toxicon23, 341–345 (1985).

    PubMed  Google Scholar 

  28. B. S. Vishwanath, R. M. Kini and T. V. Gowda,Characterization of three edema-inducing phospholipase A2 enzymes from habu (Trimeresurus flavoviridis) venom and their interaction with the alkaloid aristolochic acid. Toxicon25, 501–515 (1987).

    PubMed  Google Scholar 

  29. H. S. Seliestre, L. S. Queiroz, O. A. B. Cunha, G. E. P. De Souza and J. R. Giglio,Isolation and characterization of hemorrhagic, myonecrotic and edema-inducing toxins from Bothrops insularis. Toxicon28, 261–273 (1990).

    PubMed  Google Scholar 

  30. F. R. Mandelbaum, A. P. Reichl and M. T. Assakura,Some physical and biochemical characteristics of HF2 one of the hemorrhagic factors in the venom of Bothrops jararaca. Toxicon13, 109 (1975).

    Google Scholar 

  31. M. T. Assakura, A. P. Reichl and F. R. Mandelbaum,Comparison of immunological, biochemical and biophysical properties of three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca). Toxicon24, 943–946 (1986).

    PubMed  Google Scholar 

  32. F. R. Mandelbaum and O. B. Henriques,Purification and properties of Bothrops protease A. Arch. Biochem. Biophys.104, 369–375 (1964).

    PubMed  Google Scholar 

  33. M. Rocha e Silva, W. T. Beraldo and G. Rosenfeld,Bradykinin, a hypotensive and smooth muscle stimulating factor released from plasma globulin by snake venoms and by trypsin. Am. J. Physiol.156, 261–273 (1949).

    Google Scholar 

  34. D. Haworth, J. R. M. Heron and F. Carey,Rat paw hyperalgesia and oedema in response to NMDA Naja naja phospholipase A 2. Br. J. Pharmacol.93, 145 (1988).

    Google Scholar 

  35. S. A. Minton,Polyvalent antivenin, in the treatment of experimental snake venom poisoning. Am. J. Trop. Med.3, 1077–1082 (1954).

    Google Scholar 

  36. M. Homma and A. T. Tu,Antivenin for the treatment of local tissue damage due to envenomation by southeast Asian snake. Ineffectiveness in the prevention of local tissue damage in mice after envenomation. Am. J. Trop. Med. Hyg.19, 880–884 (1970).

    PubMed  Google Scholar 

  37. E. Martén,The surgical treatment of snake bite. InToxins: Animal. Plant and Microbiol. (Ed. P. Rosenberg) p. 417, Pergamon Press, Oxford 1978.

    Google Scholar 

  38. B. Lomonte, L. Cerdas, J. A. Gené and J. M. Gutiérrez,Neutralization of local effects of the terciopelo (Bothrops asper) venom by blood serum of the colubrid snake Clelia clelia. Toxicon20, 571–579 (1982).

    PubMed  Google Scholar 

  39. H. Moussatché, F. Leonardi and F. Mandelbaum,Inhibición por una proteína aislada del suero de Didelphis marsupialis a la acción hemorrágica por una fracción del veneno de Bothrops jararaca. Acta Cient. Venezolana32, 173 (1981).

    Google Scholar 

  40. J. G. Soto, J. C. Perez and S. A. Minton,Proteolytic, hemorrhagic and hemolytic activities of snake venoms. Toxicon26, 875–882 (1988).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Escuela de Medicina, Universidad Centro Occidental, Barquisimeto, Venezuela

    Jonas Perales

  2. Departamento de Fisiologia e Farmacodinâmica, Fundação Oswaldo Cruz, Avenida Brasil 4365, Caixa postal 926, CEP: 20040, Rio de Janeiro, Brazil

    Claudia Z. Amorim, Surza L. G. Rocha & Haity Moussatché

  3. Departamento de Bioquímica, Instituto de Química UFRJ, 21910, Rio de Janeiro, Brazil

    Gilberto B. Domont

Authors
  1. Jonas Perales
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claudia Z. Amorim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Surza L. G. Rocha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gilberto B. Domont
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Haity Moussatché
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perales, J., Amorim, C.Z., Rocha, S.L.G. et al. Neutralization of the oedematogenic activity of Bothrops Jararaca venom on the mouse paw by an antibothropic fraction isolated from Opossum (Didelphis Marsupialis) serum. Agents and Actions 37, 250–259 (1992). https://doi.org/10.1007/BF02028117

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02028117

Keywords

Search

Navigation