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Radioimmunoassay for quantifying the cytokininscis-zeatin andcis-zeatin riboside and its application to xylem sap samples

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Abstract

An antiserum against the cytokinincis-zeatin riboside was raised in rabbits and characterized for use in radioimmunoassays. Cross-reactivity studies demonstrated the specificity of the selected antiserum forcis-zeatin riboside andcis-zeatin in preference to a range of cytokinins and other purines. HPLC systems were developed that separatedcis-zeatin andcis-zeatin riboside from zeatin/dihydrozeatin and zeatin riboside/dihydrozeatin riboside, respectively. These systems enabled the separation of these compounds in xylem sap samples of wheat and oats and their quantification using radioimmunoassay. A TLC system for the separation ofcis-zeatin andcis-zeatin riboside from zeatin/dihydrozeatin and zeatin riboside/dihydrozeatin riboside, respectively, is also described.

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Authors and Affiliations

  1. Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, 2601, Canberra, ACT, Australia

    C. W. Parker, J. Badenoch-Jones & D. S. Letham

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  1. C. W. Parker
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  2. J. Badenoch-Jones
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  3. D. S. Letham
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Parker, C.W., Badenoch-Jones, J. & Letham, D.S. Radioimmunoassay for quantifying the cytokininscis-zeatin andcis-zeatin riboside and its application to xylem sap samples. J Plant Growth Regul 8, 93–105 (1989). https://doi.org/10.1007/BF02025277

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  • DOI: https://doi.org/10.1007/BF02025277

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