Abstract
Leucocytes were isolated by pronase digestion from granuloma tissue at different stages of inflammation induced by carrageenin-soaked sponge implants in rats and cyclic AMP was measured in these cells. In a mixed cell suspension, containing granulocytes and macrophages, the cyclic AMP levels increased during the early stages of inflammation but decreased when the granuloma became established. However, after correction for the proportion of infiltrating macrophages, as the inflammation progressed only a fall in cyclic AMP content was observable. Exposure of granuloma-derived cells to PGE2 resulted in a rise in cyclic AMP content, which was more pronounced in cells isolated during a later rather than an earlier stage of granuloma development. The results provide support for the earlier proposal that the anti-inflammatory effect of E-type prostaglandins on granulomas is partially explicable on the basis of cyclic AMP changes in infiltrating macrophages.
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References
I.L. Bonta andM.J. Parnham, Time-Dependent Stimulatory and Inhibitory Effects of Prostaglandin E1 on Exudative and Tissue Components of Granulomatous Inflammation in Rats, Br. J. Pharmac.65, 465–472 (1979).
M.J. Parnham, I.L. Bonta andM.J.P. Adolfs, Distinction Between Prostaglandin E2 and Prostacyclin as Inhibitors of Granulomatous Inflammation, J. Pharm. Pharmac.31, 565–567 (1979).
M.J. Parnham, M.J.P. Adolfs andI.L. Bonta, Alteration of Granuloma Formation by PGE1: Effects of an Adenylate Cyclase Inhibitor and Splenectomy, in:Advances in Prostaglandin and Thromboxane Research, (Eds. B. Samuelsson, P.W. Ramwell and R. Paoletti; Raven Press, New York 1980), pp. 1713–1716.
I.L. Bonta, M.J.P. Adolfs andM.J. Parnham,Distribution and Further Studies on the Activity of Prostaglandin E in Chronic Granulomatous Inflammation, in:Prostaglandins and Inflammation (Eds. K.D. Rainsford and A.W. Ford-Hutchinson), Agents and Actions, Suppl. 6 (Birkhäuser Verlag, Basel 1979), pp. 121–132.
O.H. Lowry, N.J. Rosebrough, A.L. Farr andR.J. Randall,Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem.193, 265–275 (1951).
A.G. Gilman,Protein Binding Assay for Adenosine-3′,5′-cyclic Monophosphate, Proc. natl. Acad. Sci. USA67, 305–312 (1970).
L.M. Pelus, H.E. Broxmeyer, J.I. Kurland andM.A.S. Moore,Regulation of Macrophage and Granulocyte Proliferation. Specificities of Prostaglandin E and Lactoferrin, J. exp. Med.150, 277–292 (1979).
R.L. Oropeza-Rendon, V. Speth, G. Hiller, K. Weber andH. Fischer, Prostaglandin E1 Reversibly Induces Morphological Changes in Macrophages and Inhibits Phagocytosis, Exp. Cell Res.119, 365–371 (1979).
R.J. Bonney, I. Gery, T.Y. Lin, M.F. Meyenhofer, W. Acevedo andP. Davies,Mononuclear Phagocytes from Carrageenan-Induced Granulomas. Isolation, Cultivation and Characterization, J. exp. Med.148, 261–275 (1978).
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Adolfs, M.J.P., Bonta, I.L. & Parham, M.J. Cyclic AMP in macrophages from experimental granulomas and the effect of prostaglandin E2 . Agents and Actions 10, 561–563 (1980). https://doi.org/10.1007/BF02024166
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DOI: https://doi.org/10.1007/BF02024166