Summary
Radiation-induced aggregation of protein in aqueous solutions was studied. Different concentrations of casein solutions have been irradiated as such or in the presence of carbohydrate and/or lipid. Gel chromatography data indicated a close relation between the amounts of aggregates and protein concentration. Protein aggregation increased with the increase in radiation dose level. The addition of carbohydrate (trehalose) reduced the amount of radiation-induced aggregates, whereas the sole addition of oil caused an induction in protein aggregation when the solution was irradiated at 1 Mrad. However, insignificant changes in protein aggregation were observed when emulsified oil was added to the casein-trehalose solution.
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Delincée, H., B. Radola: γ-Irradiation of aqueous solutions of Horseradish Peroxidase in different atmospheres: Argon Air, and Nitrous oxide. Radiat. Env. Biophys.11, 213 (1974).
Delincée, H., B. Radola. The effect of γ-irradiation on the charge and size properties of Horseradish Peroxidase. Radiat. Res.58, 9 (1974).
Delincée, H., B. Radola: Effect of γ-irradiation on the charge and size properties of Horseradish Peroxidase: Individual Isoenzymes. Radiat. Res.59, 572 (1974).
Delincée, H., V. Jakubick. The effect of carbohydrates and lipids on the radiation-induced aggregation of proteins. Intern. J. Appl. Radiat. Isotopes28, 939 (1977).
Diehl, J. F., H. Scherz: Estimation of radiolytic products as a basis for evaluating the wholesomeness of irradiated foods. Intern. J. Appl. Radiat. Isotopes26, 499 (1975).
Elias, P. S., A. J. Cohen (Eds.), Radiation chemistry of major food components. Elsevier (Amsterdam 1977).
Harmuth-Hoene, A. E., H. Delincée: Changes in the nutritive value of Casein irradiated and stored in the presence of lipids. Internat. J. Vit. Nutr. Res.48, 62 (1978).
Henrikson, T., T. B. Nelo, G. Saxebol: Radiation damage in proteins. In: Free Radicals in Biology,Pryor, W. A. Ed. Vol. II. p. 213 (New York, N.Y. 1976).
Josephson, E. S., M. H. Thomas, W. K. Calhoun: Nutritional aspects of food irradiation: An Overview. Proceeding: First Int. Cong. on Eng. and Food, Aug. 9–13, 1976. Boston, Mass.
Mee, L. K., S. J. Adelstein, G. Stein: Inactivation of Ribonuclease by the primary aqueous radicals. Radiat. Res.52, 588 (1972).
Merritt, C., P. Angelini, M. L. Bazinet, D. J. McAdoo: Irradiation damage in lipids. Adv. Chem. Ser.56, 225 (1966).
Merritt, C., P. Angelini, E. Wierbicki, W. Shults: Chemical changes associated with flavor in irradiated meat. J. Agr. Food Chem.23, 1037 (1975).
Merritt, C. M., P. Angelini, R. A. Graham: Effect of radiation parameters on the formation of radiolysis products in meat and meat substances. J. Agr. Food Chem.26, 29 (1978).
Radola, B. J.: Thin-layer gel filtration of proteins. I. Method, J. Chromatogr.38, 61 (1968).
Simic, M. G.: Radiation chemistry of amino acids and peptides in aqueous solutions. J. Agr. Food Chem.26, 6 (1978).
Tajima, M., M. Morita, M. Fujimaki: Radiation chemistry of foods. Part III: Radiolysis of Sulphur-Containing amino acids in aqueous Glucose solution. Agr. Biol. Chem.33, 1277 (1969).
Taub, I. A., R. A. Kaprielian, J. W. Halliday: Radiation chemistry of high protein foods irradiated at low temperature. Int. Symp. on Food preservation by irradiation. Vol. I, pp. 371, IAEA (Vienna 1978).
Umemoto, Y., H. Ito, Y. Sato: Effects of γ-irradiation on milk and milk proteins. Part I. Changes in physicochemical properties of whole casein by γ-irradiation and its effects on lactose-containing whole casein solutions. J. Agric. Chem. Soc. Japan,40, 271 (1966).
Wierbicki, E., A. Brynjolfsson, H. C. Johnson, D. B. Rowley: Preservation of meats by ionizing radiation — an update: Proceedings 21st European meeting of meat research workers, Rapporteurs papers, No. 14, Aug. 31–Sept. 5, Bern, Switzerland (1975).
Yousri, R. M., A. E. Harmuth-Hoene: The nutritional value of irradiated casein-fat mixtures — effect of anaerobic conditions, storage time, added vitamin E and degree of unsaturation of lipids. Internat. J. Vit. Nutr. Res.49, 171 (1979).
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Yousri, R.M. Protein aggregation in aqueous casein solution; effect of irradiation, dose level, concentration, storage, and additives (carbohydrate and lipid). Z Ernährungswiss 19, 111–121 (1980). https://doi.org/10.1007/BF02021395
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DOI: https://doi.org/10.1007/BF02021395