Summary
Horseradish peroxidase has beenγ-irradiated in dilute aqueous solution under argon, air, and nitrous oxide. The results indicate that the OH⋅ radicals are of primary importance for the radiation inactivation of peroxidase. By thin-layer isoelectric focussing a shift in the distribution of enzyme activity towards lower isoelectric points was detected in the order argon < air < N2O. Thin-layer gel chromatography revealed enzymatically active aggregates, the formation of which could be attributed mainly to the OH⋅ radicals.
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Delincée, H., Radola, B.J. γ-Irradiation of aqueous solutions of horseradish peroxidase in different atmospheres: Argon, air, and nitrous oxide. Radiat Environ Biophys 11, 213–218 (1974). https://doi.org/10.1007/BF01323190
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DOI: https://doi.org/10.1007/BF01323190