Skip to main content
SpringerLink
Log in

Enzyme-linked immunosorbent assay for detection and typing of herpes simplex virus

  • Original Articles
  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

An enzyme-linked immunosorbent assay for herpes simplex virus was tested using commercially available peroxidase-conjugated and unconjugated rabbit antibodies to herpes simplex virus type 1 and type 2 (DAKO immunoglobulins A/S, Copenhagen, Denmark). One hundred and thirty-seven clinical specimens from vesicles and superficial cutaneous lesions were tested and the results compared with virus isolation. In addition 210 herpes simplex virus isolates were typed. Forty-four specimens yielded herpes simplex virus both in the enzyme-linked immunosorbent assay and in tissue culture and 79 were negative in both tests. Fourteen were positive in isolation but negative in the enzyme-linked immunosorbent assay. Seventy-three isolates were typed as herpes simplex virus type 1 and 137 as herpes simplex virus type 2. The enzyme-linked immunosorbent assay was found to be rapid and easy to perform but less sensitive than conventional isolation in routine diagnostic work. For typing of isolates it was found to be a useful method for distinguishing herpes simplex virus type 1 and type 2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jeansson, S.: Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis. Applied Microbiology 1972, 24: 96–100.

    PubMed  Google Scholar 

  2. Geder, L., Skinner, G. R. B.: Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescent technique. Journal of General Virology 1971, 12: 179–182

    PubMed  Google Scholar 

  3. Forghani, B., Schmidt, N. H., Lennette, E. H.: Solid-phase radioimmunoassay for identification of herpes virus hominis types 1 and 2 from clinical materials. Applied Microbiology 1974, 28: 661–667.

    PubMed  Google Scholar 

  4. Mills, K. W., Gerlach, E. H., Bell, J. W., Farkas, M. E., Taylor, R. J.: Serotyping herpes simplex virus isolates by enzyme-linked immunosorbent assays. Journal of Clinical Microbiology 1978, 7: 73–76.

    PubMed  Google Scholar 

  5. Vestergaard, B. F., Jensen, O.: Diagnosis and typing of herpes simplex virus in clinical specimens by the enzyme-linked immunosorbent assay (ELISA). In: Nahmias, A. J., Dowdle, W. R., Schinazi, R. F. (ed.): The human herpesviruses. An interdisciplinary perspective. Elsevier, New York, 1981, 0. 391–394.

    Google Scholar 

  6. Sarkkinen, H. K., Tuokko, H., Halonen, P. E.: Comparison of enzyme-immunoassay and radioimmunoassay for detection of human rotaviruses and adenoviruses from stool specimens. Journal of Virological Methods 1980, 1: 331–341.

    Article  PubMed  Google Scholar 

  7. Cleveland, P. H., Richman, D. D., Oxman, M. N., Worthen, D. M.: Rapid serological technique for typing herpes simplex viruses. Journal of Clinical Microbiology 1982, 15: 402–407.

    PubMed  Google Scholar 

  8. Pereira, L., Dondero, D. V., Gallo, D., Devlin, V., Woodie, J. D.: Serological analysis of herpe simplex virus types 1 and 2 with monoclonal antibodies. Infection and Immunity 1982, 35: 363–367.

    PubMed  Google Scholar 

  9. Richman, D. D., Cleveland, P. H., Oxman, M. N.: A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus. Journal of Medical Virology 1982, 9: 299–305.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Microbiology, Section of Virology, Karolinska Hospital, S-10401, Stockholm, Sweden

    L. Grillner & M. Landqvist

Authors
  1. L. Grillner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Landqvist
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grillner, L., Landqvist, M. Enzyme-linked immunosorbent assay for detection and typing of herpes simplex virus. Eur. J, Clin. Microbiol. 2, 39–42 (1983). https://doi.org/10.1007/BF02019921

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02019921

Keywords

Search

Navigation