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Bacterial activity at the air/water interface

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Abstract

By using substrate molecules of varying degrees of surface activity, we were able to measure some features of bacterial activity in the surface microlayers (SM) and in the subsurface (bulk) water. The fraction of active cells was determined by a combined microautoradiography-epifluorescence (ME) method. Measurements were made of14CO2 evolution to determine the rate of respiration. Results from in situ measurements showed no significant difference between fraction of active cells in the SM and in the bulk. This may be due to an exchange of bacteria between SM and bulk. This exchange was assessed by spreading a film of3H-palmitic acid on the surface and, after incubation, measuring the amount of labeled cells at the surface and in the bulk. Test bacteria showing a high accumulation at the surface also showed a low exchange between the 2 strata. When low concentrations of added14C-protein were used, the respiration measurements showed a lower value for bulk than for interface localized protein. At higher concentrations, the evolved14CO2 was the same whether the protein was mixed in the bulk or spread at the surface. When 2.4–12 ng·cm−2 of14C-palmitic acid was spread on the surface, there was a linear relation between turnover time and amount of added substrate. At higher substrate concentrations there was a deviation from the straight line. Results are discussed in terms of the unique habitat found at an interface.

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References

  1. Andrews P, Williams PJ leB (1971) Heterotrophic utilization of dissolved organic compounds in the sea. III. Measurement of the oxidation rates and concentrations of glucose and amino acids in sea water. J Mar Biol Ass UK 51:111–125

    Google Scholar 

  2. Albright LJ (1980) Photosynthetic activities of phytoneuston and phytoplankton. Can J Microbiol 26:389–392

    PubMed  Google Scholar 

  3. Baier RE, Goupil DW, Perlmutter S, King R (1974) Dominant chemical composition of seasurface films, natural slicks and foams. J Rech Atmos 8:571–600

    Google Scholar 

  4. Bell CR, Albright LJ (1982) Bacteriological investigation of the neuston and plankton in the Fraser River estuary, British Columbia. Estuarine Coastal and Shelf Sci 15:385–394

    Google Scholar 

  5. Blanchard DC, Syzdek LD (1978) Seven problems in bubble and jet drop researches. Limnol Oceanogr 23:389–400

    Google Scholar 

  6. Carlucci AF, Williams PM (1965) Concentration of bacteria from sea water by bubble scavenging. J Cons Perm Int Explor Mer 30:28–33

    Google Scholar 

  7. Crawford CC, Hobbie JE, Webb KL (1974) The utilization of dissolved free amino acids by estuarine microorganisms. Ecology 55:551–563

    Google Scholar 

  8. Crawford RL, Johnson LeeAnn, Martinson M (1982) Numbers and metabolic activities of bacteria in surface films of freshwater lakes. Abstr Ann Meet Am Soc Microbiol, N2, p 178

    Google Scholar 

  9. Dahlbäck B, Gunnarsson LÅH, Hermansson M, Kjelleberg S (1982) Microbial investigations of surface microlayers, water column, ice and sediment in the Arctic Ocean. Mar Ecol Progr Series 9, 101–109

    Google Scholar 

  10. Dahlbäck B, Hermansson M, Kjelleberg S, Norkrans B (1981) The hydrophobicity of bacteria—an important factor in their initial adhesion at the air-water interface. Arch Microbiol 128:267–270

    PubMed  Google Scholar 

  11. Dietz AS, Albright LJ, Tuominen T (1976) Heterotrophic activities of bacterioneuston and bacterioplankton. Can J Microbiol 22:1699–1709

    PubMed  Google Scholar 

  12. Fletcher M, Marshall KC (1982) Are solid surfaces of ecological significance to aquatic bacteria? In: Marshall KC (ed) Advances in microbial ecology, Vol. 6., Plenum Press, New York and London, pp 199–236

    Google Scholar 

  13. Fujioka RS, Hashimoto HH, Siwak EB, Young RHF (1981) Effect of sunlight on survival of indicator bacteria in seawater. Appl Environ Microbiol 41:690–696

    PubMed  Google Scholar 

  14. Gallagher JL (1975) The significance of the surface film in salt marsh plankton metabolism. Limnol Oceanogr 20:120–123

    Google Scholar 

  15. Gocke K (1977) Comparison of methods for determining the turnover times of dissolved organic compounds. Mar Biol 42:131–141

    Google Scholar 

  16. Graham DE, Phillips MC (1979) Proteins at liquid interfaces. I. Kinetics of adsorption and surface denaturation. J Colloid Interface Sci 70:403–414

    Google Scholar 

  17. Graham DE, Phillips MC (1979) Proteins at liquid interfaces. II. Adsorption isotherms. J Colloid Interface Sci 70:415–426

    Google Scholar 

  18. Graham DE, Phillips MC (1979) Proteins at liquid interfaces. III. Molecular structures of adsorbed films. J Colloid Interface Sci 70:427–439

    Google Scholar 

  19. Gucinski H, Goupil DW, Baier RE (1981) Sampling and composition of the surface microlayer. In Eisenreich SJ (ed) Atmospheric pollutants in natural waters. Ann Arbor Science Pub, Michigan, pp 165–180

    Google Scholar 

  20. Hardy JT (1973) Phytoplankton ecology of a temperate marine lagoon. Limnol Oceanogr 18:525–533

    Google Scholar 

  21. Harvey RW, Young LY (1980) Enrichment and association of bacteria and particulates in salt marsh surface water. Appl Environ Microbiol 39:894–899

    Google Scholar 

  22. Harvey RW, Young LY (1980) Enumeration of particle-bound and unattached respiring bacteria in the salt marsh environment. Appl Environ Microbiol 40:156–160

    Google Scholar 

  23. Hermansson M, Kjelleberg S, Korhonen TK, Stenström T-A (1982) Hydrophobic and electrostatic characterization of surface structures of bacteria and its relationship to adhesion to an air-water interface. Arch Microbiol 131:308–312

    Google Scholar 

  24. Hermansson M, Kjelleberg S, Norkrans B (1979) Interaction of pigmented wildtype and pigmentless mutant ofSerratia marcescens with lipid surface film. FEMS Microbiol Lett 6:129–132

    Google Scholar 

  25. Hoppe H-G (1977) Analysis of actively metabolizing bacterial populations with the autoradiographic method. In: Rheinheimer G (ed) Microbial ecology of a brackish water environment. Ecological studies 25. Springer-Verlag Berlin, Heidelberg, New York

    Google Scholar 

  26. Horrigan SG, Carlucci AF, Williams PM (1981) Light inhibition of nitrification in sea-surface films. J Mar Res 39:557–565

    Google Scholar 

  27. Kattner GG, Brockmann UH (1978) Fatty-acid composition of dissolved and particulate matter in surface films. Mar Chem 6:233–241

    Google Scholar 

  28. Kefford B, Kjelleberg S, Marshall KC (1982) Bacterial scavenging: utilization of fatty acids localized at a solid-liquid interface. Arch Microbiol 133:257–260

    Google Scholar 

  29. Kjelleberg S (1983) Mechanisms of bacterial adhesion at air/water interfaces. In: Fletcher MM, Savage DC (eds) Mechanisms and physiological significance of bacterial adhesion. Plenum Press, New York

    Google Scholar 

  30. Kjelleberg S, Håkansson N (1977) Distribution of lipolytic, proteolytic, and amylolytic marine bacteria between the lipid film and the subsurface water. Mar Biol 39:103–109

    Google Scholar 

  31. Kjelleberg S, Stenström TA (1980) Lipid surface films: interaction of bacteria with free fatty acids and phospholipids at the air/water interface. J Gen Microbiol 116:417–423

    Google Scholar 

  32. Kjelleberg S, Humphrey BA, Marshall KC (1982) Effect of interfaces on small, starved marine bacteria. Appl Environ Microbiol 43:1166–1172

    Google Scholar 

  33. Kjelleberg S, Lagercrantz C, Larsson TH (1980) Quantitative analysis of bacterial hydrophobicity studied by the binding of dodecanoic acid. FEMS Microbiol Lett 7:41–44

    Google Scholar 

  34. Kjelleberg S, Stenström TA, Odham G (1979) Comparative study of different hydrophobic devices for sampling lipid surface films and adherent microorganisms. Mar Biol 53:21–25

    Google Scholar 

  35. Larsson K, Odham G, Södergren A (1974) On lipid surface films on the sea. I. A simple method for sampling and studies of composition. Mar Chem 2:49–57

    Google Scholar 

  36. Marumo R, Taga N, Nakai T (1971) Neustonic bacteria and phytoplankton in surface microlayers of the equatorial waters. Bull Plankton Soc Japan 18:36–41

    Google Scholar 

  37. Meyer-Reil L-A (1978) Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl Environ Microbiol 36:506–512

    PubMed  Google Scholar 

  38. Mitamura O, Matsumoto K (1981) Uptake rate of urea nitrogen and decomposition rate of urea carbon at the surface microlayer in Lake Biwa. Verh Internat Verein Limnol 21:556–564

    Google Scholar 

  39. Norkrans B (1980) Surface microlayers in aquatic environments. In: Alexander M (ed) Advances in microbial ecology. Vol. 4. Plenum Publishing Corporation, New York, pp 51–85

    Google Scholar 

  40. Parsons TR, Strickland JDH (1962) On the production of particulate organic carbon by heterotrophic processes in seawater. Deep Sea Res 8:211–222

    Google Scholar 

  41. Passman FJ, Novitsky TJ, Watson SW (1979) Surface microlayers of the North Atlantic: microbial populations, heterotrophic and hydrocarbonoclastic activities. In: Bourquin AW, Pritchard PH (eds) Microbial degradation of pollution in marine environments, U.S. Environmental Protection Agency, EPA-600/9-79-012 Gulf-Breeze, Florida, pp 214–226

    Google Scholar 

  42. Rogers HJ (1961) The dissimilation of higher molecular weight substances. In: Gunsalus IC, Stanier RY (eds) The bacteria, Vol. 2. Academic Press, New York and London, pp 257–318

    Google Scholar 

  43. Romanenko VI, Pubienes MA, Daukshta AS (1978) Growth and activity of bacteria on the surface film of water under experimental conditions. Mikrobiologiya 47:149–157

    Google Scholar 

  44. Sieburth JMcN (1971) An instance of bacterial inhibition in oceanic surface water. Mar Biol 11:98–100

    Google Scholar 

  45. Sieburth JMcN, Willis P-J, Johnson KM, Burney CM, Lavoie DM, Hinga KR, Caron DA, French III FW, Johnson PW, Davis PG (1976) Dissolved organic matter and heterotrophic microneuston in the surface microlayers of the North Atlantic. Science 194:1415–1418

    Google Scholar 

  46. Syzdek LD (1982) Concentration ofSerratia in the surface microlayer. Limnol Oceanogr 27:172–177

    Google Scholar 

  47. Tabor PS, Neihof RA (1982) Improved microautoradiographic method to determine individual microorganisms active in substrate uptake in natural waters. Appl Environ Microbiol 44:945–953

    Google Scholar 

  48. Van Es FB, Meyer-Reil L-A (1982) Biomass and metabolic activity of heterotrophic marine bacteria. In: Marshall KC (ed) Advances in microbial ecology, Vol. 6. Plenum Press, New York and London, pp 111–170

    Google Scholar 

  49. Väätänen P (1976) Microbiological studies in coastal waters of the Northern Baltic Sea. I. Distribution and abundance of bacteria and yeasts in the Tvärminne area. Walter and Andrée de Nottbeck Found Scient Rep 1:1–58

    Google Scholar 

  50. Williams PJ leB, Berman T, Holm-Hansen O (1976) Amino acid uptake and respiration by marine heterotrophs. Mar Biol 35:41–47

    Google Scholar 

  51. Wright RT (1978) Measurement and significance of specific activity in the heterotrophic bacteria of natural waters. Appl Environ Microbiol 36:297–305

    Google Scholar 

  52. Wright TT, Hobbie JE (1966) Use of glucose and acetate by bacteria and algae ecosystems. Ecology 47:447–464

    Google Scholar 

  53. ZoBell CE (1943) The effect of solid surfaces upon bacterial activity. J Bacteriol 46:39–54

    Google Scholar 

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Authors and Affiliations

  1. Department of Marine Microbiology, Botanical Institute, University of Göteborg, Carl Skottsbergs Gata 22, S-41319, Göteborg, Sweden

    Malte Hermansson & Björn Dahlbäck

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  1. Malte Hermansson
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  2. Björn Dahlbäck
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Hermansson, M., Dahlbäck, B. Bacterial activity at the air/water interface. Microb Ecol 9, 317–328 (1983). https://doi.org/10.1007/BF02019021

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