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Effects of sera on bone resorption in tissue culture

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Abstract

Unheated-normal rabbit and rat sera stimulate bone resorption in tissue culture. The magnitude of this effect is enhanced when the sera were incubated with an antigen-antibody precipitate. Sera heated prior to treatment with antigen-antibody precipitate had no effect on bone resorption. The stimulation of bone resorption by sera was associated with osteoclast formation. Our results are consistent with a heat labile factor in some sera which stimulates bone resorption. Whether this factor is a component of complement or not is not answered by our studies.

Résumé

Du sérum non chauffé normal de lapin et de rat stimule la résorption osseuse en culture de tissu. Cet effet est augmenté, lorsque le sérum est incubé avec un précipité antigène-anticorps. Du sérum chauffé avant traitement par le précipité antigène-anticorps n'a pas d'effet sur la résorption osseuse. La stimulation de la résorption osseuse par le sérum est associée à une apparition d'ostéoclastes. Ces résultats semblent indiquer l'existence d'un facteur sensible à la chaleur dans certains sérums, qui stimule la résorption osseuse. Il n'a pas pu être établi si ce facteur est un constituent du complément.

Zusammenfassung

Unerhitztes, normales Serum von Kaninchen und Ratten regt die Knochenresorption in der Gewebekultur an. Diese Wirkung wird erhöht, wenn das Serum mit einem Antigen-Antikörper-Niederschlag inkubiert wurde. Serum, welches vor der Behandlung mit Antigen-Antikörper-Niederschlag erhitzt wurde, hatte keine Wirkung auf die Knochenresorption. Die Stimulation der Knochenresorption durch Serum ging mit Osteoclastenbildung einher. Unsere Ergebnisse stimmen mit der Annahme überein, daß ein hitzelabiler Faktor in einigen Seren vorkommt, welcher die Knochenresorption anregt. Unsere Untersuchungen geben keinen Aufschluß darüber, ob dieser Faktor ein Bestandteil des Komplementes ist oder nicht.

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Authors and Affiliations

  1. Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 4510 Main Street, 14226, Buffalo, New York, USA

    E. Hausmann, R. Genco, N. Weinfeld & R. Sacco

Authors
  1. E. Hausmann
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  2. R. Genco
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  3. N. Weinfeld
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  4. R. Sacco
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Additional information

This research was supported by U. S. Public Health Service Grants DE 01932, DE 02814, and DE 00167.

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Hausmann, E., Genco, R., Weinfeld, N. et al. Effects of sera on bone resorption in tissue culture. Calc. Tis Res. 13, 311–317 (1973). https://doi.org/10.1007/BF02015421

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  • DOI: https://doi.org/10.1007/BF02015421

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