Abstract
The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost) and a latex agglutination test (Rotalex). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex, 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost, 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex.
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Morinet, F., Ferchal, F., Colimon, R. et al. Comparison of six methods for detecting human rotavirus in stools. Eur. J, Clin. Microbiol. 3, 136–140 (1984). https://doi.org/10.1007/BF02014331
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DOI: https://doi.org/10.1007/BF02014331