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Enzyme immunoassay for detection of antigen in acutePlasmodium falciparum malaria

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Abstract

A monoclonal antibody specific for an epitope of a 50 kDa Plasmodium falciparum antigen was used in an enzyme immunoassay for detection of the corresponding exo-antigen in culture supernatant and in the sera of 31 patients suffering from acute malaria. The assay was specific for Plasmodium falciparum and did not appear to be strain restricted. A parasitaemia level below 0.001 % could be detected.

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Authors and Affiliations

  1. Unité 42 INSERM, 369 rue Jules Guesde, 59650, Villeneuve, d'Ascq, France

    B. Fortier, P. Delplace & J. F. Dubremetz

  2. Faculté de Médecine, Laboratoire de Parasitologie, 1 Place Verdun, 59045, Lille Cédex, France

    F. Ajana

  3. BioMérieux, Chemin de l'Orme, Marcy l'Etoile, 69752, Charbonnières les Bains Cédex, France

    A. Vernes

Authors
  1. B. Fortier
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  2. P. Delplace
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  3. J. F. Dubremetz
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  4. F. Ajana
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  5. A. Vernes
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Fortier, B., Delplace, P., Dubremetz, J.F. et al. Enzyme immunoassay for detection of antigen in acutePlasmodium falciparum malaria. Eur. J, Clin. Microbiol. 6, 596–598 (1987). https://doi.org/10.1007/BF02014260

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  • DOI: https://doi.org/10.1007/BF02014260

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