Skip to main content
SpringerLink
Log in

Purification and properties of glutamine synthetase I fromRhizobium sp. UMKL 20

  • Short Communications
  • Anatomy, Physiology
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Glutamine synthetase I was purified fromRhizobium sp. UMKL 20 following polyethylene glycol precipitation. The enzyme had a subunit molecular weight of 58 kd. Apparent Km values for ammonia and glutamate were 5.6 and 15.2 mM, respectively. Glutamine synthetase I activity was inhibited by several end products of glutamine metabolism. The purified enzyme was highly adenylylated (E −n =8.5).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Stadtman, E. R., and Ginsberg, A., EnzymesX (1970) 755.

    Google Scholar 

  2. Darrow, R. A., and Knotts, R. R., Biochim. biophys. Res. Commun.78 (1977) 554.

    Article  CAS  Google Scholar 

  3. Fuchs, R. L., and Keister, D. L., J. Bact.144 (1980) 641.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Yap, S. L., and Lim, S. T., Archs Microbiol.135 (1983) 224.

    Article  CAS  Google Scholar 

  5. Yelton, M. M., Yang, S. S., Edie, S., and Lim, S. T., J. gen. Microbiol.129 (1983) 1537.

    Google Scholar 

  6. Shapiro, B. M., and Stadtman, E. R., Meth. Enzym.17A (1970) 910.

    Article  Google Scholar 

  7. Prusiner, S., and Milner, L., Analyt. Biochem.37 (1970) 429.

    Article  CAS  PubMed  Google Scholar 

  8. Streicher, S. L., and Tyler, B. J., J. Bact.142 (1980) 69.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Laemmli, U. K., Nature, Lond.227 (1970) 680.

    Article  CAS  PubMed  Google Scholar 

  10. Donohue, T. J., and Bernlohr, R. W., J. Bact.147 (1981) 589.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Meyer, J. M., and Stadtman, E. R., J. Bact.146 (1981) 705.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Stollar, D., and Levine, L., Meth. Enzym.VI (1963) 848.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Malaya, Lembah Pantai, Kuala Lumpur, Malaysia

    S. T. Lim

Authors
  1. S. T. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. I would like to thank Mr J. C. Lai for technical assistance. This work was carried out with the support of Vote F 153/79 from the University of Malaya.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lim, S.T. Purification and properties of glutamine synthetase I fromRhizobium sp. UMKL 20. Experientia 41, 727–728 (1985). https://doi.org/10.1007/BF02012567

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02012567

Key words

Search

Navigation