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The characterization of amidohydrolases in a freshwater lake sediment

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Abstract

The properties of three amidohydrolases, i.e., urease (I) EC 3.5.1.5, L-asparaginase (II) EC 3.5.1.1, and L-glutaminase (III) EC 3.5.1.2, were studied in sediment samples taken from a shallow eutrophic freshwater lake.

Sediment samples were air dried (ADS) and stored for at least 3 months before being enzymically characterized. The pH optimum of I, II, and III were pH 7.0, 8.4, and 6.5–7.0, respectively, while III in soluble extracts from ADS was most active between pH 8.0 and 9.0. The temperature response of the three enzymes in ADS gave Ea values of 38.9, 41.6, and 35.9 kJmol−1 for I, II, and III, respectively. Km and Vmax values for ADS I, II, and III were 1.2 mM and 1.9μmol NH3 g−1h−1; 0.8 mM and 4.1μmol NH3 g−1h−1; and 1.25 mM and 17.4μmol NH3 g−1h−1. Km values for all three enzymes in ADS extracts were at least an order of magnitude greater than those of the ADS. The susceptability of each enzyme to proteolysis was followed in ADS and fresh wet sediment and compared with that of III in an ADS extract. All sediment enzymes were found to be more resistant than the commercial preparation of bacterial L-glutaminase subjected to the same treatment. These results suggested that I, II, and III all exist to some extent as colloid-immobilized enzyme fractions in freshwater sediments and are analogous to the stable enzyme fractions in soils.

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References

  1. Billen G (1982) Modelling the processes of organic matter degradation and nutrients recycling in sedimentary systems. In: Nedwell DB, Brown CM (eds) Sediment microbiology. Academic Press, London, pp 15–52

    Google Scholar 

  2. Bremner JM, Mulvaney RL (1978) Urease activity in soils. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 149–196

    Google Scholar 

  3. Bullock P, Loveland PJ (1974) Mineralogical analyses. In: Avery BW, Bascomb CL (eds) Soil survey laboratory methods. Harpenden Press, Rothamsted, pp 57–69

    Google Scholar 

  4. Burns RG (1978) Enzyme activity in soil: Some theoretical and practical considerations. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 295–340

    Google Scholar 

  5. Burns RG (1982) Enzyme activity in soil: Location and a possible role in microbial ecology. Soil Biol Biochem 14:423–427

    Article  Google Scholar 

  6. Chandramohan D, Devandran K, Natarajan R (1974) Arylsulphatase activity in marine sediments. Mar Biol 27:89–92

    Article  Google Scholar 

  7. Dalal RC (1975) Effect of toluene on the energy barriers of urease activity of soils. Soil Sci 120:256–260

    Google Scholar 

  8. Dharmaraj K, Selvakumar N, Chandramohan D, Natarajan R (1977) L-Glutaminase activity in marine sediments. Indian J Mar Sci 6:168–170

    Google Scholar 

  9. Duddridge JE, Wainwright M (1982) Enzyme activity and kinetics in substrate-amended river sediments. Water Res 16:329–334

    Article  Google Scholar 

  10. Frankenberger WT, Johanson JB (1983) Amidohydrolase activities in natural waters. Pol Arch Hydrobiol 30:319–329

    Google Scholar 

  11. Frankenberger WT, Page AL (1983) The effects of acidity and alkalinity on the stability of amidohydrolases in freshwater. J Envir Qual 12:459–462

    Google Scholar 

  12. Galstyan ASh, Saakyan EG (1973) Determination of soil L-glutaminase activity. Dolk Akad Nauk SSSR 209:1201–1202

    Google Scholar 

  13. Gould WD, Cook FD, Webster GR (1973) Factors affecting urea hydrolysis in several Alberta soils. Pl Soil 38:393–401

    Article  Google Scholar 

  14. Jones JG (1979) Microbial nitrate reduction in aquatic sediments. J Gen Microbiol 115:27–35

    Google Scholar 

  15. Jones JG (1982) Activities of aerobic and anaerobic bacteria in lake sediments and their effect on the water column. In: Nedwell DB, Brown CM (eds) Sediment microbiology. Academic Press, London, pp 107–145

    Google Scholar 

  16. Kiss S, Dragan-Bularda M, Radulescu D (1975) Biological significance of enzymes accumulated in soils. Adv Agron 27:25–87

    Google Scholar 

  17. Lea PJ, Fowden L, Miflin BJ (1978) Purification and properties of Asparaginase fromLupinus sp. Phytochem 17:217–222

    Article  Google Scholar 

  18. Levin Y, Pecht M, Goldstein L, Katchalski E (1964) A water-insoluble polyanionic derivative of trypsin. I. Preparation and properties. Biochem 3:1905–1913

    Article  Google Scholar 

  19. McLaren AD, Babcock KL (1959) Some characteristics of enzyme reactions at surfaces. In: Hayashi T (ed) Subcellular particles. Ronald Press, New York, pp 23–35

    Google Scholar 

  20. Meyer-Reil LA (1981) Enzymatic decomposition of proteins and carbohydrates in marine sediments: Methodology and field observations during sampling. Kieler Meeresforsch Sonderh 5:311–317

    Google Scholar 

  21. Mortimer CH (1941) The exchange of dissolved substances between mud and water in lakes (Parts I and II). J Ecol 29:280–329

    Google Scholar 

  22. Mouraret M (1965) L-Asparaginase activity in soil. Memoires, Orstom, Paris

    Google Scholar 

  23. Omura H, Sato F, Hayano K (1983) A method for estimating L-glutaminase activity in soils. Soil Sci Pl Nutr (Tokyo) 29:295–303

    Google Scholar 

  24. Paulson KN, Kurtz LT (1970) Michaelis constant of soil urease. Soil Sci Soc Am J 34:70–72

    Google Scholar 

  25. Perucci P, Guisquiani PL, Scarponi L (1982) Nitrogen losses from added urea and urease activity of a clay-loam soil amended with crop residues. Pl Soil 69:457–463

    Google Scholar 

  26. Pettit NM, Smith ARJ, Freedman RB, Burns RG (1976) Soil urease: Activity, stability and kinetic properties. Soil Biol Biochem 8:479–484

    Article  Google Scholar 

  27. Pulford ID, Tabatabai MA (1988) Effect of waterlogging on enzyme activities in soils. Soil Biol Biochem 20:215–219

    Article  Google Scholar 

  28. Radulescu D, Kiss S (1971) Enzyme activities in the sediments of the Zanoaga and Zanoguta lakes (Retezat mountain mass). In: Progress in Roumanian palinology. Ed Acad Rep Soc Rom, Bucharest, pp 243–248

    Google Scholar 

  29. Satoh Y (1981) Decomposition of urea by a size-fractionated planktonic community in a eutrophic reservoir in Japan. Hydrobiologia 83:153–160

    Google Scholar 

  30. Selvakumar N, Chandramohan D, Natarajan R (1977) L-Asparaginase activity in marine sediments. Curr Sci 46:287–291

    Google Scholar 

  31. Sepers BJ (1981) Diversity of ammonifying bacteria. Hydrobiologia 83:343–350

    Article  Google Scholar 

  32. Skiba U, Wainwright M (1983) Assay and properties of some sulphur enzymes in coastal sands. Pl Soil 70:125–132

    Google Scholar 

  33. Tabatabai MA (1973) Michaelis constant of urease in soils and soil fractions. Soil Sci Soc Am Proc 37:707–710

    Google Scholar 

  34. Wade HE (1980) Synthesis and functions of microbial asparaginases and glutaminases. In: Payne JW (ed) Microorganisms and nitrogen sources: Transport and utilization of amino acids, peptides, proteins and related substances. Wiley Interscience, Chichester, pp 563–575

    Google Scholar 

  35. Zantua MI, Bremner JM (1975) Comparison of methods of assaying urease activity in soils. Soil Biol Biochem 7:291–295

    Article  Google Scholar 

  36. Zantua MI, Bremner JM (1977) Stability of urease in soils. Soil Biol Biochem 9:135–140

    Article  Google Scholar 

  37. Zeikus JG (1983) Metabolic communication between biodegradative populations in nature. In: Slater JH, Wittenbury R, Wimpenny JW (eds) Microbes in their natural environments. Cambridge University Press, Cambridge, pp 423–462

    Google Scholar 

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  1. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, UK

    Paul J. Sallis & Richard G. Burns

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  1. Paul J. Sallis
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  2. Richard G. Burns
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Sallis, P.J., Burns, R.G. The characterization of amidohydrolases in a freshwater lake sediment. Microb Ecol 17, 159–170 (1989). https://doi.org/10.1007/BF02011850

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