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Isolation of bovine eosinophils and characterization of their leukotriene formation

  • Allergy, Histamine and Kinins
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Abstract

A method for the isolation of eosinophils from bovine blood is described. Eosinophils were prepurified in a simple centrifugation step and subsequently separated on a Percoll gradient into two populations with densities of 1.088 and 1095 g/cm3, respectively, and a purity of greater than 90%. No apparent differences could be observed in electron micrographs and most of the granules appeared homogenous.

A minor fraction of granules appeared to have a lamellated crystalline core structure. Eosinophils of bovine blood generate leukotriene B4 and its stereoisomers but not sulfidopeptide leukotrienes (slow-reacting substance) when stimulated by the Ca-ionophore A23187.

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  1. Institute of Biochemistry, University of Fribourg, Rue du museé 5, CH-1700, Fribourg, Switzerland

    J. Freiburghaus &  A. Jörg

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  1. J. Freiburghaus
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  2. A. Jörg
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Freiburghaus, J., Jörg, A. Isolation of bovine eosinophils and characterization of their leukotriene formation. Agents and Actions 31, 16–22 (1990). https://doi.org/10.1007/BF02003216

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  • DOI: https://doi.org/10.1007/BF02003216

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