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Saponaria vaccaria ‘Pink Beauty’, a new test plant for carnation mottle virus

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Abstract

Saponaria vaccaria ‘Pink Beauty’ was found to be a test plant for carnation mottle virus. Its value was compared with that ofChenopodium amaranticolor for indexing for the virus, and this bioassay was compared with a serological diagnosis. Serological double-diffusion tests gave the quickest reactions, but proved to be the least sensitive; addition of 1M urea did not increase sensitivity. Local lesions resulting from infectivity tests on detached leaves ofC. amaranticolor in a climate room could be counted after 1 week and these tests were about equally sensitive as those on leaves on intact plants of this species in the glasshouse, which were read after about 10 days. Infectivity tests onSaponaria vaccaria ‘Pink Beauty’ resulted in systemic symptoms 10 to 14 days after inoculation; this method appeared to be the most sensitive.

Samenvatting

Na de ontdekking, datSaponaria vaccaria ‘Pink Beauty’ gevoelig is voor het vlekkerigheidsvirus van anjer, werd de waarde van de toetsplantenmethoden met deze en andere indicatorplanten onderling vergeleken en met de gebruikelijke serologische toets voor dit virus. De serologische dubbel-diffusiemethode leverde het snelst resultaten op, maar was het minst gevoelig. Ook toevoeging van 1M ureum aan het medium vergrootte deze gevoeligheid niet. Inoculatie van losse bladeren vanChenopodium amaranticolor in een klimaatkamer leverde lokale lesies op, welke na een week konden worden geteld. Deze methode was vrijwel even betrouwbaar als toetsing op bladeren aan intacte planten van dezelfde soort in de kas, welke reacties na 10 dagen afgelezen konden worden. De traagste, maar gevoeligste methode was de infectietoets metS. vaccaria ‘Pink Beauty’ die ook waarde heeft voor het aantonen van enkele virussen, waarvoorC. amaranticolor niet vatbaar is.

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Stationed at the Experiment Station for Floriculture in the Netherlands, Aalsmeer, the Netherlands.

This work was carried out during a stay of the student Miss van Olphen at the Experiment Station at Aalsmeer, the Netherlands.

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Hakkaart, F.A., van Olphen, M. Saponaria vaccaria ‘Pink Beauty’, a new test plant for carnation mottle virus. Netherlands Journal of Plant Pathology 77, 127–133 (1971). https://doi.org/10.1007/BF02000552

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  • DOI: https://doi.org/10.1007/BF02000552

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