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The anti-inflammatory effect of glucocorticoid-induced phospholipase inhibitory proteins

  • General Inflammation
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Abstract

The anti-inflammatory effect of glucocorticoids has been investigated in two standard models of experimental inflammation, i.e. rat paw oedema induced by carrageenin or dextran.

Both types of oedema are suppressed by dexamethasone while indomethacin and BW755C only suppress carrageenin oedema.

Dexamethasone inhibits dextran oedema according to the accepted mode of action of steriod hormones since the inhibition occurs after a 2–3 h time lag and is abolished by pretreating animals with actinomycin D. Dextran oedema and carrageenin oedema are also controlled by endogenous corticoids since adrenalectomy potentiates the paw oedema formation induced by low concentrations of phlogogenic agents.

It has been shown that glucocorticoids induce bothin vitro andin vivo the formation and release of antiphospholipase proteins which are anti-inflammatory in that they greatly suppress carrageenin oedema. However, these proteins have no effect on dextran oedema.

We conclude that the inhibition of dextran oedema by glucocorticoids depends on the formation of another type of anti-inflammatory protein.

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References

  1. R. Carnuccio, M. Di Rosa andP. Persico,Hydrocortisone induced inhibitor of prostaglandin biosynthesis in rat leucocytes, Br. J. Pharmac.68, 14–16 (1980).

    Google Scholar 

  2. G.J. Blackwell, R. Carnuccio, M. Di Rosa, R.J. Flower, L. Parente andR. Persico,Macrocortin: a polypeptide causing the anti-phospholipase effect of glucocorticoids,Nature, Lond. 287, 147–149 (1980).

    Google Scholar 

  3. F. Hirata, E. Schiffman, K. Venkatasubramanian, D. Salomon andJ. Axelrod,A phospholipase A 2 inhibitory protein in rabbit neutrophils induced by glucocorticoids. Proc. Natn. Acad. Sci. USA77, 2533–2536 (1980).

    Google Scholar 

  4. J.F. Cloix, O. Colard, B. Rothhut andF. Russo-Marie,Characterization and partial purification of ‘renocortins’: two polypeptides formed in renal cells causing the anti-phospholipase-like action of glucocorticoids, Br. J. Pharmac.79, 313–321 (1983).

    Google Scholar 

  5. F. Hirata, Y. Notsu, M. Iwata, L. Parente, M. Di Rosa andR.J. Flower,Identification of several species of phospholipase inhibitory protein(s) by radioimmunoassay for lipomodulin, Biochem. biophys. Res. Commun.109, 223–230 (1983).

    Article  Google Scholar 

  6. G.J. Blackwell, R. Carnuccio, M. Di Rosa, R.J. Flower, C.S.J. Langham, L. Parente, P. Persico, C. Russell-Smith andD. Stone,Glucocorticoids induce the formation and release of anti-inflammatory and anti-phospholipase proteins into the peritoneal cavity of the rat. Br. J. Pharmac.76, 185–194 (1982).

    Google Scholar 

  7. G.J. Blackwell, R. Carnuccio M. Di Rosa, R.J. Flower, J. Ivanyi, C.S.J. Langham, L. Parente, P. Persico andJ. Wood, Suppression of archidonate oxidation by glucocorticoid-induced anti-phospholipase peptides. InProstaglandins, Thromboxane and Leukotriene Res., Vol. 11, pp. 65–71 (EdsB. Samuelsson, R. Paoletti andP. Ramwell). Raven Press, New York 1983.

    Google Scholar 

  8. M. Di Rosa andD.A. Willoughby,Screens for anti-inflammatory drugs, J. Pharm. Pharmac.23, 297–298 (1971).

    Google Scholar 

  9. G.A. Higgs, R.J. Flower andJ.R. Vane,A new approach to anti-inflammatory drugs, Biochem. Pharmac.28, 1959–1961 (1979).

    Article  Google Scholar 

  10. R.E. Buller andB.W. O'Malley,The biology and mechanism of steroid hormone receptor interaction with the eukaryotic nucleus, Biochem. Pharmac.25, 1–12 (1976).

    Article  Google Scholar 

  11. S. Tsurufuji, K. Sugio andF. Takemasa,The role of glucocorticoid receptor and gene expression in the anti-inflammatory action of dexamethasone, Nature, Lond.280, 408–410 (1979).

    Google Scholar 

  12. Y. Oyanagui,Steroid-like anti-inflammatory effect of superoxide dismutase in serotonin, histamine, and kinin-induced edemata of mice: existence of vascular permeability regulating proteins(s), Biochem. Pharmac.30, 1791–1798 (1981).

    Article  Google Scholar 

  13. Y. Oyanagui,Anti-inflammatory effects of polyamines in serotonin and carrageenan paw edemata—possible mechanism to increase vascular permeability inhibitory protein level which is reulated by glucocorticoids and superoxide radical, Agents and Actions14, 81–89 (1984).

    Google Scholar 

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Authors and Affiliations

  1. Department of Experimental Pharmacology, University of Naples, via L. Rodino 22, 80138, Naples, Italy

    A. Calignano, R. Carnuccio, M. Di Rosa & A. Ialenti

  2. Department of Prostaglandin Research Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK

    S. Moncada

Authors
  1. A. Calignano
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  2. R. Carnuccio
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  3. M. Di Rosa
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  4. A. Ialenti
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  5. S. Moncada
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Calignano, A., Carnuccio, R., Di Rosa, M. et al. The anti-inflammatory effect of glucocorticoid-induced phospholipase inhibitory proteins. Agents and Actions 16, 60–62 (1985). https://doi.org/10.1007/BF01999650

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  • DOI: https://doi.org/10.1007/BF01999650

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