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Kinetic studies of embryo development and nutrient utilization in an alfalfa direct somatic embryogenic system

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Abstract

A method for direct somatic embryogenesis in alfalfa (Medicago falcata) is described. The time course in the development phase has been followed for fresh weight, cell density, pH, sugar uptake and embryo number and type. The method of disrupting the explant material has also been shown to influence subsequent embryo formation.

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Abbreviations

ABA:

abscisic acid

B5:

medium of Gamborg et al. (1968)

COT:

cotyledon state embryos

2.4-D:

2,4-dichlorophenoxyacetic acid

FW:

fresh weight

MS:

medium of Murashige & Skoog (1962)

PEG:

polyethylene glycol

POLY:

polyembryos

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Authors and Affiliations

  1. Institute of Genetic Engineering, 2232, Kostinbrod-2, Bulgaria

    Plamen D. Denchev, Alexander I. Kuklin, Atanas I. Atanassov & Alan H. Scragg

  2. The University of the West of England, BS16 1QY, Frenchay, Bristol, UK

    Alan H. Scragg

Authors
  1. Plamen D. Denchev
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  2. Alexander I. Kuklin
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  3. Atanas I. Atanassov
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  4. Alan H. Scragg
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Denchev, P.D., Kuklin, A.I., Atanassov, A.I. et al. Kinetic studies of embryo development and nutrient utilization in an alfalfa direct somatic embryogenic system. Plant Cell Tiss Organ Cult 33, 67–73 (1993). https://doi.org/10.1007/BF01997600

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  • DOI: https://doi.org/10.1007/BF01997600

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