Abstract
Explants from new growth of forced dormant stems and ‘secondary cultures’ of Vanhoutte's spirea were cultured on Linsmaier and Skoog (L.S.) medium containing benzyladenine (BA), indoleacetic acid (IAA), thidiazuron (TDZ), and zeatin. The dormant stems were forced by immersing their basal portions in forcing solutions containing 626 µM 8-hydroxyquinoline citrate (8-HQC) and 2% sucrose. BA and gibberellic acid (GA3) were also added into the forcing solutions to determine if explants obtained from the new growth will benefit from this treatment when culturedin vitro.
L.S. medium supplemented with 5 µM BA alone, 5 µM BA plus 1 or 5 µM IAA, and 0.5 or 0.75 µM TDZ alone produced the best shoot proliferation for both sources of explants. BA and GA3 appeared to be taken up from the forcing solution by the new softwood growth. BA in the forcing solution stimulatedin vitro shoot proliferation in different degrees depending on the period of treatment, while GA3 caused lessin vitro shoot proliferation. It is proposed that forcing solutions containing plant growth regulators (P.G.R.) are a useful approach for manipulating responses of plant tissues culturedin vitro.
Similar content being viewed by others
References
Briggs BA, McCulloch SM & Edick LA (1988) Micropropagation of azaleas using thidiazuron. Acta Hort. 226: 205–208
Economou AS & Read PE (1982) Effect of NAA on shoot productionin vitro from BA-pretreated petunia leaf explants. J. Amer. Soc. Hort. Sci. 107(3): 504–506
Economou AS & Read PE (1986) Microcutting production from sequential reculturing of hardy deciduous azalea shoot tips. HortScience 21(1): 137–139
Gavinlertvatana P, Read PE, Wilkins HF & Heins R (1979) Influence of photoperiod and daminozide stock plant pretreatments on ethylene and CO2 levels and callus formation from dahlia leaf segment cultures. J. Amer. Soc. Hort. Sci. 104(6): 849–852
Herrington E (1988) Red light induced shoot proliferation in cultures ofSpiraea nipponica. In Vitro 24(3): 34A, Abstract #91
Linsmaier EM & Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127
Marousky FJ (1968) Physiological role of 8-hydroxyquinoline citrate and sucrose in extending vase life and improving quality of cut gladiolus. Proc. Fla. State Hort. Soc. 81: 409–414
Norton ME & Boe AA (1982)In vitro propagation of ornamental Rosaceous plants. HortScience 17(2): 190–191
Norton ME & Norton CR (1986)In vitro shoot proliferation ofPrunus andSpiraea in relation to explant type. The Plant Propagator 32 (3): 5
Norton CR & Norton ME (1988a) Manipulation of differentiation in spiraeain vitro. Acta Hort. 226: 191–194
Norton ME & Norton CR (1988b) Stock plant conditioning, the generation of propagative material and propagule selection in spiraea. Acta Hort. 226: 369–374
Preece JE, Zhao J & Kung FH (1989) Callus production and somatic embryogenesis from white ash. HortScience 24(2): 377–380
Read PE, Economou AS & Fellman CD (1984) Manipulating stock plants for improvedin vitro mass propagation. In: Novak FJ, Havel L & Dolezel J (Eds) Application to Crop Improvement, Proc. Int. Symp. Plant Tissue and Cell Culture. Czech. Acad. Sci. Prague: 467–473
Read PE, Gavinlertvatana P, Suriyajantratong P, Garton S & Brenner M (1978) Stock plants affect tissue culture success. In: Hughes K, Henke R & Constantin M (Eds) Propagation of higher plants through tissue culture. Univ. of Tennessee, Knoxville (p. 249)
Read PE & Yang Q (1987) Novel plant growth regulator delivery systems forin vitro culture of horticultural plants. Acta Hort. 212: 55–59
Read PE & Yang G (1989a) Influencing propagation by stock plant PGR treatments. Acta Hort. 251: 121–127
Read PE & Yang G (1989b) Responsein vitro of explants chemically treated via forcing solutions. Proc. Int. Plant Prop. Soc. Vol. 38: 406–408
Skoog F & Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues culturedin vitro. Symp. Soc. for Exp. Biol. 11: 118–131
Tjia B, Marousky FJ & Stamps RH (1987) Response of cut gerbera flowers to fluoridated water and floral preservative. HortScience 22(5): 896–897
Yang G, Read PE & Yue J (1988) Effects of BA and IAA on Vanhoutte's spiraea propagatedin vitro. HortScience 23(3), sec. 2: 130
Yang G & Read PE (1989) Effects of BA, GA3, IAA and NAA in the forcing solution on bud break and shoot elongation in forced woody stems. Proc. Plant Growth Regulator Soc. Amer. 150–155
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yang, G., Read, P.E. In vitro culture of vanhoutte's spirea explants from ‘secondary cultures’ and dormant stems forced in solutions containing plant growth regulators. Plant Cell Tiss Organ Cult 33, 25–30 (1993). https://doi.org/10.1007/BF01997594
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01997594