Nitroprusside, a nitrogen oxide generating drug, inhibits release of histamine and tryptase from human skin mast cells
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We have investigated the effects of the nitric oxide donor sodium nitroprusside on the immunologic release of histamine and tryptase from human mast cells. Isolated human skin mast cells were purified using Percoll gradient centrifugation. Mast cells with a purity of 70–90% were preincubated for 30 minutes with sodium nitroprusside and subsequently stimulated with anti-IgE. Upon challenge with anti-IgE, a release of 6.37±0.46 nmol histamine and 612±49 ng tryptase per 106 cells was observed. In presence of sodium nitroprusside a dose-dependent decrease of the release occurred. The results suggest that nitric oxide is effective in reducing the release of histamine and tryptase from human skin mast cells.
KeywordsOxide Nitrogen Nitric Oxide Mast Cell Histamine
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