Summary
Cadmium has a singular ability to promote the functional reconstitution of tissue factor (coagulation factor III) apoprotein with vesicles formed from mixed bovine brain lipids. Cadmium causes rapid release of carboxyfluorescein from lipid vesicles which chromatograph in the included volume of Bio Gel A5m, and also promotes reconstitution of tissue factor with these vesicles. With larger vesicles, which are excluded on Bio Gel A5m, cadmium produces only low levels of reconstitution and leakage. Calcium has been shown to promote only low levels of tissue factor-vesicle reconstitution, and produces only low levels of carboxyfluorescein leakage when compared to cadmium. These results demonstrate that cadmium and calcium interact differently with the small mixed lipid vesicles, and suggest that the cadmium-promoted reconstitution of tissue factor vesicle-complexes accompanies cadmium-induced alterations in vesicle structure. The results also show that cadmium affects large and small vesicles differently.
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References
Ackers, G.K. 1964. Molecular exclusion and restricted diffusion processes in molecular-seive chromatography.Biochemistry 3:723–730
Bach, R., Nemerson, Y., Konigsberg, W. 1981. Purification and characterization of bovine tissue factor.J. Biol. Chem. 256:8324–8331
Carson, S.D., Konigsberg, W.H. 1980a. Cadmium increases tissue factor (coagulation factor III) activity by facilitating its reassociation with lipids.Science 208:307–309
Carson, S.D., Konigsberg, W.H. 1980b. Lipid activation of coagulation factor III apoprotein (tissue factor) — Reconstitution of the protein-membrane complex.Thromb. Haemostas. 44:12–15
Carson, S.D., Konigsberg, W.H. 1981. Coagulation factor III (tissue factor) interaction with phospholipid vesicles induced by cadmium: characterization of the reconstituted proteinmembrane complex.Biosci. Rep. 1:197–205
Huang, C.-H. 1969. Studies on phosphatidylcholine vesicles. Formation and physical characteristics.Biochemistry 8:344–352
Ingolia, T.D., Koshland, D.E., Jr. 1978 The role of calcium in fusion of artificial vesicles.J. Biol. Chem. 253:3821–3829
Liao, M.-J., Prestegard, J.H. 1979. Fusion of phosphatidic acidphosphatidylcholine mixed lipid vesicles.Biochim. Biophys. Acta 550:157–173
Liao, M.-J., Prestegard, J.H. 1980. Ion specificity in fusion of phosphatidic acid-phosphatidylcholine mixed lipid vesicles.Biochim. Biophys. Acta 601:453–461
Liao, M.-J., Prestegard, J.H. 1981. Structural properties of a Ca2+-phosphatidic acid complex. Small angle X-ray scattering and calorimetric results.Biochim. Biophys. Acta 645:149–156
Macala, L.J., Yu, R.K. 1982. High-performance thin-layer chromatography densitometry of brain lipids.Trans. Am. Soc. Neurochem. 13:147
Nemerson, Y. 1968. The phospholipid requirement of tissue factor in blood coagulation.J. Clin. Invest. 47:72–80
Papahadjopoulos, D., Portis, A. 1978. Calcium-induced phase transitions and membrane fusion.Ann. N.Y. Acad. Sci. 308:50–63
Papahadjopoulos, D., Vail, W.J., Newton, C., Nir, S., Jacobson, K., Poste, G., Lazo, R. 1977. Studies on membrane fusion. III. The role of calcium-induced phase changes.Biochim. Biophys. Acta 465:579–598
Pitlick, F.A., Nemerson, Y. 1976. Purification and characterization of tissue factor apoprotein.Methods Enzymol. 45:37–48
Weinstein, J.N., Yoshikami, S., Henkart, P., Blumenthal, R., Hagins, W.A. 1977. Liposome-cell interaction: Transfer and intracellular release of a trapped fluorescent marker.Science 195:489–492
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Carson, S.D. Cadmium causes vesicle leakage under conditions which favor reconstitution of tissue factor-vesicle complexes. J. Membrain Biol. 75, 123–127 (1983). https://doi.org/10.1007/BF01995632
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DOI: https://doi.org/10.1007/BF01995632