Abstract
Coagulation factor III (tissue factor) is a membrane glycoprotein which serves as a cofactor in the proteolytic activation of factor X and factor IX by factor VIIa. Mixing of human placental factor III apoprotein with vesicles of bovine brain phospholipids does not produce significant reconstitution of factor III activity, but, when the mixture of apoprotein and vesicles is made 5 mM with CdCI2, the apoprotein is incorporated into the vesicles. Ultracentrifugation on sucrose density gradients demonstrated that the active factor III-lipid complex formed by reconstitution with vesicles had a density indistinguishable from that of the complex formed by detergent dialysis. Vesicles isolated after centrifugation were shown to range in diameter from 20 nm to over 100 nm using the electron microscope. Gel filtration showed that factor-III activity was associated with all size-classes of vesicles. The presence of factor III activity in the smaltest vesicles argues for a specific cadmium-mediated reconstitution of the apoprotein with phospholipid vesicles.
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References
Pitlick FA & Nemerson Y (1970)Biochemistry 9, 5105–5113.
Nemerson Y (1966)Biochemistry 5, 601–608.
Osterud B & Rapaport SI (1977)Proc. Natl. Acad. Sci. U.S.A. 74, 5260–5264.
Carson SD & Konigsberg WH (1980)Science 208, 307–309.
Carson SD & Konigsberg WH (1980)Thrombos. Haemostas. 44, 12–15.
Nemerson Y (1968)J. Clin. Invest. 47, 72–80.
Hvatum M & Prydz, H (1969)Thrombos. Diathes. Haemorrh. 21, 217–222.
Nemerson Y (1969)J. Clin. Invest. 48, 322–331.
Wijngaards G, van Deenen LLM, & Hemker HC (1977)Biochim. Biophys. Acta 488, 161–171.
Nemerson Y, Zur M, Bach R, & Gentry R (1980) inRegulation of Coagulation (Mann KB and Taylor FB, eds), pp 193–202, Elsevier, New York.
Pitlick FA & Nemerson Y (1978)Meth. Enzymol. 45, 37–48.
Chen PS Jr, Toribara TY, & Warner H (1956)Anal. Chem. 28, 1756–1758.
Bartlett GR (1959)J. Biol. Chem. 234, 466–469.
Godson GN, Fiddles JC, Barrel BG, & Sanger F (1978) inThe Single-Stranded DNA Phages (Denhardt DT, Dressler D, & Ray DS, eds), pp 51–86, Cold Spring Harbor.
Godson GN (1978) inThe Single-Stranded DNA Phages (Denhardt DT, Dressler D, and Ray DS, eds), pp 103–112, Cold Spring Harbor.
Huang C-H (1969)Biochemistry 8, 344–352.
Liao M-J & Prestegard JH (1979)Biochim. Biophys. Acta 550, 157–173.
Holz RW & Stratford CA (1979)J. Membrane Biol. 46, 331–358.
Papahadjopoulos D, Vail WJ, Pangborn WA, & Poste G (1976)Biochim. Biophys. Acta 448, 265–283.
Nelsestuen GL, Kisiel W, & DiScipio RG (1978)Biochemistry 17, 2134–2138.
Tanford C (1980)The Hydrophobic Effect, 2nd ed, pp 114–117, John Wiley and Sons, New York.
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Carson, S.D., Konigsberg, W.H. Coagulation factor III (tissue factor) interaction with phospholipid vesicles induced by cadmium: characterization of the reconstituted protein-membrane complex. Biosci Rep 1, 197–205 (1981). https://doi.org/10.1007/BF01114905
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DOI: https://doi.org/10.1007/BF01114905