Skip to main content
Log in

The effect of nitric oxide generators on ischemia reperfusion injury and histamine release in isolated perfused guinea-pig heart

  • Histamine Release from Mast Cells and Basophils
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Experiments were carried out to provide evidence of the effect ofl-arginine (l-Arg), its analogue NG-monomethyl-l-arginine (MeArg) and of some nitrovasodilators (sodium nitroprusside, NaNP; 3-morpholino-sydnonimine, SIN-1) which spontaneously release nitric oxide (NO) on ischemia-reperfusion injury, histamine release and mast cell degranulation, occurring after multiple ligature and release of the left anterior descending (LAD) coronary artery in isolated perfused guinea-pig hearts. The reopening of the LAD coronary artery leads to a release of histamine related to a decrease in microdensitometry of cardiac mast cells and to calcium overload. The perfusion of the heart with NO-donors significantly reduces either the release of histamine, the loss of mast cell metachromasia and the overload of calcium. These effects were potentiated by SOD. The results suggest that the endogenous formation of NO and molecules able to generate NO have a role in the prevention of post-ischemic tissue injury.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. R. F. Furchgott,The role of endothelium in the responses of vascular smooth muscle to drugs. Ann. Rev. Pharmacol. Toxicol.24, 175–197 (1984).

    Google Scholar 

  2. L. J. Ignarro, G. M. Buga, K. S. Wood, R. E. Byrns and G. Caudhuri,Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. USA84, 9265–9269 (1987).

    PubMed  Google Scholar 

  3. R. M. J. Palmer, D. D. Rees, D. S. Ashton and S. Moncada,l-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochem. Biophys. Res. Commun.153, 1251–1255 (1988).

    PubMed  Google Scholar 

  4. P. F. Mannaioni, E. Masini, A. Pistelli, D. Salvemini and J. R. Vane,Rat mast cells inhibit platelet aggregation by releasing a nitric oxide-like factor: influence of histamine release. InNew perspectives in histamine research (Ed. H. Timmerman) Birkhäuser, Basel, 1990, in press.

    Google Scholar 

  5. K. Rydzynski, H. Dalen, T. Saetersdal and H. Engedal,Morphologic and morphometric analysis of the mast cells from human heart biopsies. Agents and Actions20, 288–290 (1987).

    PubMed  Google Scholar 

  6. E. Masini, S. Bianchi, F. Gambassi, B. Palmerani, A. Pistelli, L. Carlomagno and P. F. Mannaioni,Ischemic-reperfusion injury and histamine release in isolated and perfused guinea-pig heart: pharmacological interventions. Agents and Actions30, 198–201 (1990).

    PubMed  Google Scholar 

  7. A. M. Lefer, P. S. Tsao, D. J. Lefer and G. Johnson,Time course and mechanism of inhibition of coronary EDRF release during myocardial ischemia and reperfusion. Arch. Int. Pharmacodyn.305, 262 (1990).

    Google Scholar 

  8. M. Feelisch and E. Noak,Correlation between nitric oxide formation during degradation of organic nitrates and activation of guanylate cyclase. Eur. J. Pharmacol.139, 19–30 (1987).

    PubMed  Google Scholar 

  9. T. L. Brunton,Amylnitrate in angina pectoris. Lancet2, 97 (1867).

    Google Scholar 

  10. A. M. Keller, R. M. Clancy, M. L. Barr, C. C. Marbue and P. J. Cannon,Acute reoxygenation injury in isolated rat heart: role of resident cardiac mast cells. Circulation Res.63, 1044–1052 (1988).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Masini, E., Bianchi, S., Mugnai, L. et al. The effect of nitric oxide generators on ischemia reperfusion injury and histamine release in isolated perfused guinea-pig heart. Agents and Actions 33, 53–56 (1991). https://doi.org/10.1007/BF01993125

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01993125

Keywords

Navigation