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Effect of histamine on the mitogenic response of human lymphocytes and its modification by cimetidine and levamisole

  • Histamine and Immunology
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Abstract

The phytohaemagglutinin (PHA) mitogenic response of human blood lymphocytes demonstrated a dose-dependent inhibition by histamine (in the range 5×10−7 M). Using suboptimal mitogenic doses of PHA the suppression was more pronounced. Stimulation using different doses of pokeweed mitogen (PWM) was not altered by histamine. Purified T cells and a lymphocyte population depleted of histamine-receptor-bearing cells were less sensitive to the histamine effect. In the supernatants of histamine-stimulated cultures, suppressor factor activity was found. Cimetidine could reverse the effect of histamine in a dose-dependent manner. Adding levamisole to the PHA cultures also produced some antihistaminic effect.

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Authors and Affiliations

  1. National Institute of Rheumatology and Physiotherapy, Frankel Leo u. 17-19, 1027, Budapest, Hungary

    K. Meretey

  2. The Mathilda and Terence Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, W6 7DW, London, UK

    G. Room & R. N. Maini

Authors
  1. K. Meretey
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  2. G. Room
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  3. R. N. Maini
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Meretey, K., Room, G. & Maini, R.N. Effect of histamine on the mitogenic response of human lymphocytes and its modification by cimetidine and levamisole. Agents and Actions 11, 84–88 (1981). https://doi.org/10.1007/BF01991465

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  • DOI: https://doi.org/10.1007/BF01991465

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