Abstract
Induction of a calcium-independent nitric oxide synthase (NOS) has been detected in the rat small intestine and colon, 3–5 h following endotoxin (3 mg kg−1 i.v.). This was associated with an increase in vascular permeability to plasma protein in jejunum and colon, and both induction of NOS and vascular leakage were inhibited by pretreatment with dexamethasone (1 mg kg−1 s.c.). Induction of colonic NOS was also detected in a TNB colitis model, 24 h after challenge. Thus, increased NO synthesis may be involved in the microvascular and mucosal injury associated with inflammatory diseases of the gut.
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References
I. R. Hutcheson, B. J. R. Whittle and N. K. Boughton-Smith,Role of nitric oxide in maintaining vascular integrity in endotoxin-induced acute intestinal damage in the rat. Br. J. Pharmacol.101, 815–820 (1990).
N. K. Boughton-Smith, I. R. Hutcheson, A. M. Deakin, B. J. R. Whittle and S. Moncada,Protective effect of S-nitroso-N-acetyl-pencillamine in endotoxin-induced acute intestinal damage in the rat. Eur. J. Pharmacol.191, 485–488 (1990).
M. W. Radomski, R. M. J. Palmer and S. Moncada,Glucocorticoids inhibit the expression of an inducible, but not the constitutive, nitric oxide synthase in vascular endothelial cells. Proc. Natl. Acad. Sci. USA87, 10043–10047 (1990).
R. M. J. Palmer, L. Bridge, N. A. Foxwell and S. Moncada,The role of nitric oxide in endothelial cell damage and its inhibition by glucocorticoids. Br. J. Pharmacol.105, 11–12 (1992).
N. K. Boughton-Smith, J. L. Wallace, G. P. Morris and B. J. R. Whittle,The effects of anti-inflammatory drugs on eicosanoid formation in a chronic model of inflammatory bowel disease in the rat. Br. J. Pharmac.94, 65–72 (1988).
N. K. Boughton-Smith, J. L. Wallace and B. J. R. Whittle,Relationship between arachidonic acid metabolism, myeloperoxidase activity and leukocyte infiltration in a rat model of inflammatory bowel disease. Agents and Actions25, 115–123 (1988).
M. Salter, R. G. Knowles and S. Moncada,Widespread tissue distribution, species distribution and changes in activity of Ca 2+ -dependent and Ca 2+ -independent nitric oxide synthases. FEBS.291, 145–149 (1991).
J. F. Brown, B. L. Tepperman, P. J. Hanson, B. J. R. Whittle and S. Moncada,Differential distribution of nitric oxide synthase between cell fractions isolated from the rat gastric mucosa. Biochem. Biophys. Res. Commun.184, 680–685 (1992).
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Boughton-Smith, N.K., Evans, S.M., Whittle, B.J.R. et al. Induction of nitric oxide synthase in rat intestine and its association with tissue injury. Agents and Actions 38 (Suppl 2), C125–C126 (1993). https://doi.org/10.1007/BF01991159
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DOI: https://doi.org/10.1007/BF01991159