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The effect of WEB 2086 on PAF-induced eosinophil chemotaxis and LTC4 production from eosinophils

  • Allergy and Histamine
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Abstract

We investigated the effect of WEB 2086, a selective platelet-activating factor (PAF) receptor antagonist, on PAF-induced eosinophil chemotaxis and LTC4 production. WEB 2086 inhibited PAF-induced eosinophil chemotaxis in normals and asthmatics. To further determine if WEB 2086 is a selective PAF receptor antagonist, we examined the effect of WEB 2086 against formyl-methionyl-leucyl-phenylalanine (fMLP)-induced or eosinophil chemotactic factor of anaphylaxis (ECF-A)-induced eosinophil chemotaxis. WEB 2086 did not have a significant inhibition against fMLP or ECF-A-induced eosinophil chemotaxis. These results suggest that WEB 2086 is a selective and potent inhibitor of PAF-induced eosinophil chemotaxis and LTC4 production from eosinophils, due to its antagonism of PAF-receptors.

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Abbreviations

PAF:

platelet activating factor

LTC4 :

leukotriene C4

fMLP:

formyl-methionyl-leucyl-phenylalanine

ECF-A:

eosinophil chemotactic factor of anaphylaxis

HBSS:

Hank's balanced salt solution

HPF:

high power field

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Authors and Affiliations

  1. Allergic Disease Center, Creighton University School of Medicine, 2500 California Street, 68178, Omaha, NE, USA

    H. Miyagawa, M. Nabe, R. J. Hopp, C. Okada, A. K. Bewtra & G. Townley

Authors
  1. H. Miyagawa
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  2. M. Nabe
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  3. R. J. Hopp
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  4. C. Okada
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  5. A. K. Bewtra
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  6. G. Townley
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Miyagawa, H., Nabe, M., Hopp, R.J. et al. The effect of WEB 2086 on PAF-induced eosinophil chemotaxis and LTC4 production from eosinophils. Agents and Actions 37, 39–43 (1992). https://doi.org/10.1007/BF01987888

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  • DOI: https://doi.org/10.1007/BF01987888

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