Abstract
The effect of hydration andpH on the thermal stability of proteinase K was studied in the temperature range 310–450 K by differential scanning calorimetry. The dependences of the denaturation temperatureT d, the specific enthalpy of denaturation ΔH d and the maximum of excess apparent specific heat capacityC maxex upon the degree of hydrationh and thepH of the buffers used are presented. The relation betweenT d andh is of the Flory-Garrett's type. By means of Ooi's model, the two components of the denaturation enthalpy arising from hydration and conformational change, respectively, were estimated. The fact that the specific denaturation enthalpy of proteinase K is very low may be attributed to its very low enthalpy of conformational change per heavy atom.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
C. Betzel, G. P. Pal and W. Saenger, Eur. J. Biochem., 178 (1988) 155.
B.-N. Wang and F. Tan, Chinese J. Biochem. Biophys., 25 (1992) 213.
B.-N. Wang and F. Tan, Acta Biochim. Biophys. Sinica (in Chinese), 22 (1990) 189.
J. M. Sturtevant, Ann. Rev. Phys. Chem., 38 (1987) 463.
P. L. Privalov, Advances in Protein Chemistry, Academic Press, New York 1982, p. 1.
P. J. Flory and R. R. Garrett, J. Am. Chem. Soc., 80 (1958) 4836.
M. Rüegg, U. Moor and B. Blanc, Biochim. Biophys. Acta, 400 (1975) 334.
M. Luescher, M. Rüegg and P. Schindler, Biopolymers, 13 (1974) 2489.
C. Betzel, G. P. Pal and W. Saenger, Acta Crystallogr., Sect. B: Struct. Sci., 44 (1988) 163.
J. A. Dean (ed.), Lange's Handbook of Chemistry, McGraw-Hill Book Company, New York 1985 (13th ed.), P. 10–91.
L. P. Witnauer and J. G. Fee, J. Polymer Sci., 26 (1957) 141.
P. L. Privalov and N. N. Khechinashvili, J. Mol. Biol., 86 (1974) 665.
F. A. Gunkel and G. Gassen, Eur. J. Biochem., 179 (1989) 185.
M. Oobatake and T. Ooi, Prog. Biophys. Mol. Biol., 59 (1993) 237.
J. K. Dattagupta, T. Fujiwara, E. V. Grishin et al., J. Mol. Biol., 97 (1975) 267.
W. Ebeling, N. Hennrich and M. Klochow, Eur. J. Biochem., 47 (1974) 91.
J. Bajorath, W. Saenger and G. P. Pal, Biochim. Biophys. Acta, 954 (1988) 176.
J. P. Dick and A. Nordwig, Arch. Biochem. Biophys., 117 (1966) 466.
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Menachem Steinberg on the occasion of his 65th birthday
This major project was supported by the National Natural Science Foundation of China.
Rights and permissions
About this article
Cite this article
Bangning, W., Buxing, H. & Fu, T. Effect of hydration andpH on the thermal stability of proteinase K. Journal of Thermal Analysis 50, 73–80 (1997). https://doi.org/10.1007/BF01979550
Issue Date:
DOI: https://doi.org/10.1007/BF01979550