Abstract
We are currently characterizing different macrophage sub-populations according to their release of oxygen free radicals (measured as chemiluminescence) under various stimuli and their responses to drugs and mediators. Mouse macrophages were obtained by peritoneal or lung lavage and stimulatedin vitro with opsonized zymosan (OpZ) or the calcium ionophore A23187. Chemiluminescence was measured in a luminometer and resonses obtained after 5 min expressed as percentages of standard responses to the stimuli. Almost identical dose-response curves to OpZ were obtained with peritoneal and alveolar macrophages, while the dose-response curve of peritoneal macrophages to A23187 was much shallower. The response to A23187 was generally much weaker than that to OpZ and in both types of macrophages PGE2 was a more effective inhibitor of OpZ-induced chemiluminescence than of that inducd by A23187. The response to OpZ in peritoneal macrophages was inhibited in a dose-related manner by both PGE2 and superoxide dismutase, but not by indomethacin,d-penicillamine, piroxicam or phenylbutazone. OpZ but not A23187, is a suitable stimulus for pharmacological studies on macrophage chemiluminescence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J.R. Oehler, R.B. Herberman andH.T. Holden,Modulation of immunity by macrophages, Pharmac. Ther. A2, 551–593 (1978).
J.L. Humes, S. Burger, M. Galavage, F.A. Kuehl, P.D. Wightman, M.E. Dahlgren, P. Davies andR.J. Bonney,The diminished production of arachidonic acid oxygenation products by elicited mouse peritoneal macrophages: possible mechanisms, J. Immunol.124, 2110–2116 (1980).
M.J.P. Adolfs, I.L. Bonta andM.J. Parnham,Cyclic AMP in macrophages from experimental granulomas and the effect of prostaglandin E 2 , Agents and Actions10, 561–563 (1980).
H. Tomioka andH. Saito,Hydrogen peroxide-releasing function of chemically elicited and immunologically activated macrophages: differential response to wheat germ lectin and concanavalin A, Infect. Immun.29, 469–476 (1980).
M.J. Weidemann, B.A. Peskar, K. Wrogemann, E.Th. Rietschel, H. Staudinger andH. Fischer,Prostaglandin and thromboxane synthesis in a pure macrophage population and the inhibition by E-type prostaglandins of chemiluminescence, FEBS Lett.89, 136–140 (1978).
J.E. Smolen andG. Weissmann,Effects of indomethacin, 5,8,11,14-eicosatetraynoic acid and p-bromophenacyl bromide on lysosomal enzyme release and superoxide anion generation by human polymorphonuclear leucocytes, Biochem. Pharmac.29, 533–538 (1980).
M.J. Weidemann, R. Smith, T. Heaney andS. Alaudeen,On the mechanism of the generation of chemiluminescence by macrophages, Behring Inst. Mitt.65, 42–54 (1980).
J.E. Leymeyer andR.B. Johnston,Effect of antiinflammatory drugs and agents that elevate intracellular cyclic AMP on the release of toxic oxygen metabolites by phagocytes: Studies in a model of tissue-bound IgG, Clin. Immunol. Immunopath.9, 482–490 (1978).
K. Van Dyke, C. Van Dyke, J. Udeinya, C. Brister andM. Wilson,A new screening system for non-steroidal anti-inflammatory drugs based upon inhibition of chemiluminescence produced from human cells (granulocytes), Clin. Chem.25, 1655–1661 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parnham, M.J., Bittner, C. & Winkelmann, J. Macrophages from different sources, their production of chemiluminescence under various stimuli and the effects of PGE2 and drugs. Agents and Actions 11, 617–619 (1981). https://doi.org/10.1007/BF01978765
Issue Date:
DOI: https://doi.org/10.1007/BF01978765