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Complementation of an alcohol dehydrogenase-deficientNicotiana plumbaginifolia mutant by transformation with theArabidopsis thaliana Adh gene

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Abstract

An alcohol dehydrogenase-deficient (ADH) mutant ofNicotiana plumbaginifolia, selected on the basis of ethanol resistance, was restored for ADH activity by transformation with anAdh gene fromArabidopsis thaliana expressed under the control of its own promoter or the CaMV 35S promoter. The expression in various organs (seed, root, leaf and pollen) was analysed at the protein and RNA levels as well as byin situ detection of ADH activity. The analysis of spatial and temporal regulation of theA. thaliana Adh gene expression suggests that ADH expression is controlled at the transcriptional level.

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Author notes

  1. P. Rousselin

    Present address: Max-Planck-Institut für Züchtungsforschung, D-50829, Köln, Germany

  2. R. Dolferus

    Present address: CSIRO Division of Plant Industry, GRO Box 1600, ACT 2601, Canberra, Australia

Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, INRA Centre de Versailles, F-78026, Versailles cedex, France

    P. Rousselin & M. Caboche

  2. Laboratorium voor Plantengenetica, Vrife Universiteit Brussel, B-1640, Genesius-Rode, Belgium

    N. Toro Perea, R. Dolferus, B. Tahar & M. Jacobs

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  1. P. Rousselin
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  2. N. Toro Perea
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  5. M. Caboche
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Rousselin, P., Perea, N.T., Dolferus, R. et al. Complementation of an alcohol dehydrogenase-deficientNicotiana plumbaginifolia mutant by transformation with theArabidopsis thaliana Adh gene. Transgenic Research 3, 376–387 (1994). https://doi.org/10.1007/BF01976769

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