Abstract
Transformed callus was produced from peanut (Arachis hypogaea L. cv. Okrun) hypocotyl explants after four days of co-cultivation withAgrobacterium tumefaciens strains EHA101, LBA4404 or ASE1 carrying the binary vector pKYLX71GUS on a defined medium followed by selection with kanamycin (200 mg l−1). Transformed calluses were cultured as independent cell lines potentially derived from a single transformation event. Stable integration and expression of foreign gene(s) in the callus was confirmed by Southern and western blot analyses and enzyme assays. A few cell lines showed a single insert of the foreign gene. Using the above protocol, transformed peanut callus expressing the peanut stripe virus coat protein gene was obtained.
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References
An, G. (1987) Binary Ti vectors for plant transformation and promoter analysis.Meth. Enzymol. 153, 292–305.
Beachy, R.N., Loesch-Fries, S. and Tumer, N.E. (1990) Coat protein-mediated resistance against virus infection.Annu. Rev. Phytopathol. 28, 451–74.
Cassidy, B., Sherwood, J.L. and Nelson, R.S., (1992) Cloning of the capsid protein gene from a blotch isolate of peanut stripe virus.Archiv. Virol. 128, 287–97.
Clemente, T.E., Robertson, D., Isleib, T.G., Beute, M.K. and Weissinger, A.K. (1992) Evaluation of peanut (Arachis hypogaea L.) leaflets from mature zygotic embryos as recipient tissue for biolistic gene transfer,Transgenic Res. 1, 275–84.
Culver, J.N. and Sherwood, J.L. (1987) Resistance to peanut stripe virus inArachis germplasm.Pl. Disease 71, 1080–2.
Demski, J.W. and Reddy, D.V.R. (1988) Peanut stripe virus yield loss studies. In Patancheru, A.P. ed.,Coordination of Research on Peanut Stripe Virus, pp. 20. India: ICRISAT.
Franklin, C.I., Trieu, T.N., Gonzales, R.A. and Dixon, R.A. (1991) Plant regeneration from seedling explants of green bean (Phaseolus vulgaris) via organogenesis.Pl. Cell, Tissue Organ Cult. 24, 199–206.
Franklin, C.I., Trieu, T.N., Cassidy, B.G., Dixon, R.A. and Nelson, R.S. (1993) Genetic transformation of green bean callus viaAgrobacterium mediated DNA transfer.Pl. Cell Rep. 12, 74–9.
Hanley-Bowdoin, L. and Hemenway, C. (1992) Expression of plant viral genes in transgenic plants. In Wilson, T.M. and Davies, J.W., eds.,Genetic Engineering with Plant Viruses, pp. 251–95. Boca Raton, Florida, USA.
Harlow, E. and Lane, D. (1988)Antibodies: a Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. (1983) A binary plant vector strategy based on separation ofvir- and T-region of theAgrobacterium tumefaciens Ti-plasmid.Nature 303, 179–80.
Hood, E.E., Helmer, G.L., Fraley, R.T. and Chilton, M.D. (1986) The hypervirulence ofAgrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.J. Bacteriol 168, 1291–301.
Jefferson, R.A. (1987) Assaying chimeric genes in plants: thegus gene fusion system.Pl. Mol. Biol. Rep. 5, 387–405.
Lacorte, C., Mansur, E., Timmerman, B. and Cordeiro, A.R. (1991) Gene transfer into peanut (Arachis hypogaea L.) byAgrobacterium tumefaciens.Pl. Cell Rep. 10, 354–7.
McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R. and Fraley, R. (1986) Leaf disc transformation of cultivated tomato (L. esculentum) usingAgrobacterium tumefaciens.Pl. Cell Rep. 5, 81–4.
McKently, A.H., Moore, G.A. and Gardner, F.P. (1991) Regeneration of peanut and perennial peanut from cultured leaf tissues.Crop Sci. 31, 833–7.
Mroginski, L.A., Kartha, K.K. and Shyluk, J.P. (1981) Regeneration of peanut plantlets byin vitro culture of immature leaves.Can. J. Bot. 59, 826–30.
Murashige, T., and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiol. Plant. 15, 473–97.
Register, J.C. and Nelson, R.S. (1992) Early events in plant virus infection: relationships with genetically engineered protection and host gene resistance.Seminars in Virology 3, 441–51.
Restrepo, M.A., Freed, D.P. and Carrington, J.C. (1990) Nuclear transport of plant potyviral proteins.Pl. Cell 2, 987–98.
Sherwood, J.L. and Melouk, H.A. (1986) A comparison of an enzyme-linked immunosorbent assay and western blotting for the detection of peanut mottle virus and peanut stripe virus.Peanut Sci. 13, 64–7.
White, J.L. and Kaper, J.M. (1989) A simple method for detection of viral satellite RNAs in small plant tissue samples.J. Virol. Meth. 23, 83–94.
Wynne, J.C. and Beute, M.K. (1991) Breeding for disease resistance in peanut (Arachis hypogaea L.)Ann. Rev. Phytopathol. 29, 279–303.
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Franklin, C.I., Shorrosh, K.M., Trieu, A.N. et al. Stable transformation of peanut callus viaAgrobacterium-mediated DNA transfer. Transgenic Research 2, 321–324 (1993). https://doi.org/10.1007/BF01976172
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DOI: https://doi.org/10.1007/BF01976172