Abstract
General studies of the bacterial pathogenXanthomonas campestris have revealed many classes of gene which are essential for pathogenicity to cruciferous plants. ‘Pathogenicity genes’ include structural genes encoding extracellular plant-degrading enzymes, genes required for the export (or secretion) of the enzymes from the bacterial cell, several independent sets of regulatory genes, a cluster ofhrp genes, and several genes of unknown function. Plants respond to infection by synthesizing characteristic pathogenesis-related proteins. The spectrum of proteins produced depends on the genotype of the challenging bacterium.Arabidopsis is susceptible to infection byXanthomonas, which should make it feasible to analyze plant resistance and response by molucular genetic techniques.
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Arlat, M., Gough, C.L., Barber, C.E., Boucher, C. & Daniels, M.J., 1991.Xanthomonas campestris contains a cluster ofhrp genes related to the largerhrp cluster ofPseudomonas solanacearum. Molecular Plant-Microbe Interactions 4: 593–601.
Barny, M.A., Guinebretière, M.H., Marcais, B., Coissac, E., Paulin, J.P. & Laurent, J., 1990. Cloning of a large gene cluster involved inErwinia amylovora CFBP1430 virulence. Molecular Microbiology 4: 777–786.
Barrère, G.C., Barber, C.E. & Daniels, M.J., 1986. Molecular cloning of genes involved in the production of the extracellular polysaccharide xanthan byXanthomonas campestris pv.campestris. International Journal of Biological Macromolecules 8: 372–374.
Beer, S.V., Bauer, D.W., Jiang, X.H., Laby, R.J., Sneath, B.J., Wei, Z.M., Wilcox, D.A. & Zumoff, C.H., 1991. Thehrp gene cluster ofErwinia amylovora. In: Hennecke, H. & Verma, D.P. (Eds), Advances in the molecular genetics of plant-microbe interactions. Kluwer, Dordrecht. p. 53–60.
Bonas, U., Schulte, R., Fenselau, S., Minsavage, G.V., Staskawicz, B.J. & Stall, R.E., 1991. Isolation of a gene cluster fromXanthomonas campestris pv.vesicatoria that determines pathogenicity and the hypersensitive response on pepper and tomato. Molecular Plant-Microbe Interactions 4: 81–88.
Boucher, C.A., Van Gijsegem, F., Barberis, P.A., Arlat, M. & Zischek, C., 1987.Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered. Journal of Bacteriology 169: 5626–5632.
Bowles, D.J., 1990. Defense-related proteins in higher plants. Annual Review of Biochemistry 59: 873–907.
Bradbury, J.F., 1986. Guide to Plant Pathogenic Bacteria. Farnham Royal, CAB International
Collinge, D.B. & Slusarenko, A.J., 1987. Plant gene expression in response to pathogens. Plant Molecular Biology 9: 389–410.
Collinge, D.B., Milligan, D.E., Dow, J.M., Scofield, G. & Daniels, M.J., 1987. Gene expression inBrassica campestris showing a hypersensitive response to the incompatible pathogenXanthomonas campestris pv.vitians. Plant Molecular Biology 8: 405–414.
Conrads-Strauch, J., Dow, J.M., Milligan, D.E., Parra, R. & Daniels, M.J., 1990. Induction of hydrolytic enzymes inBrassica campestris in response to pathovars ofXanthomonas campestris. Plant Physiology 93: 238–243.
Coplin, D.L. & Cook, D., 1990. Molecular genetics of extracellular polysaccharide biosynthesis in vascular phytopathogenic bacteria. Molecular Plant-Microbe Interactions 3: 271–279.
Daniels, M.J., 1987. Molecular genetics of pathogenicity ofXanthomonas campestris: technical aspects. In: Day, P.R. & Jellis, G.J. (Eds), Genetics and plant pathogenesis. Blackwell Scientific Publications, Oxford.
Daniels, M.J., 1988. Molecular genetics of host pathogen interactions. In: Palacios, R & Verma, D.P.S. (Eds), Proceedings of the 4th International Symposium on Molecular Genetics of Plant-Microbe Interactions. APS Press. p. 229–234.
Daniels, M.J., Barber, C.E., Turner, P.C., Sawczyc, M.K., Byrde, R.J.W. & Fielding, A.H., 1984. Cloning of genes involved in pathogenicity ofXanthomonas campestris pv.campestris using the broad host range cosmid pLAFR1. The EMBO Journal 3: 3323–3328.
Daniels, M.J., Fan, M.J., Barber, C.E., Clarke, B.R. & Parker, J.E., 1991. Interaction betweenArabidopsis thaliana andXanthomonas campestris. In: Hennecke, H. & Verma, D.P.S. (Eds), Advances in molecular genetics of plant-microbe interactions. Kluwer, Dordrecht. p. 84–89.
De Crécy-Lagard, V., Glaser, P., Lejeune, P., Sismeiro, O., Barber, C.E., Daniels, M.J. & Danchin, A., 1990. AXanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity. Journal of Bacteriology 172: 5877–5883.
Dong, X., Mindrinos, M., Davis, K.R. & Ausubel, F.M., 1991. Induction ofArabidopsis defense genes by virulent and avirulentPseudomonas syringae strains and by a cloned avirulence gene. The Plant Cell 3: 61–72.
Dow, J.M., Scofield, G., Trafford, K., Turner, P.C. & Daniels, M.J., 1987. A gene cluster inXanthomonas campestris pv.campestris required for pathogenicity controls the excretion of polygalacturonate lyase and other enzymes. Physiological and Molecular Plant Pathology 31: 261–271.
Dow, J.M., Milligan, D.E., Jamieson, L., Barber, C.E. & Daniels, M.J., 1989. Molecular cloning of a polygalacturonate lyase gene fromXanthomonas campestris pv.campestris and role of the gene product in pathogenicity. Physiological and Molecular Plant Pathology 35: 113–120.
Dow, J.M., Clarke, B.R., Milligan, D.E., Tang, J.-L. & Daniels, M.J., 1990. Extracellular proteases fromXanthomonas campestris pv. campestris, the black rot pathogen. Applied and Environmental Microbiology 56: 2994–2998.
Dickson, M.D. & Hunter, J.E., 1987. Inheritance of resistance in cabbage seedlings to black rot. Horticultural Science 22: 108–109.
Dums, F., Dow, J.M. & Daniels, M.J., 1991. Structural characterization of protein secretion genes of the bacterial phytopathogenXanthomonas campestris pathovarcampestris: relatedness to secretion systems of other gram-negative bacteria. Molecular and General Genetics 229: 357–364.
Estelle, M.A. & Somerville, C.R., 1986. The mutants ofArabidopsis. Trends in Genetics 2: 89–93.
Feldman, K.A., 1991. T-DNA insertion mutagenesis inArabidopsis: mutational spectrum. The Plant Journal 1: 71–82.
Gough, C.L., Dow, J.M., Barber, C.E. & Daniels, M.J., 1988. Cloning of two endoglucanase ofXanthomonas campestris pv.campestris: analysis of the role of the major endoglucanase in pathogenesis. Molecular Plant-Microbe Interactions 1: 275–281.
Gough, C.L., Dow, J.M., Keen, J., Henrissat, B. & Daniels, M.J., 1990. Nucleotide sequence of theengXCA gene encoding the major endoglucanase ofXanthomonas campestris pv.campestris. Gene 89: 53–59.
Harding, N.E., Cleary, J.M., Cabanas, D.K., Rosen, I.G. & Kang, K.S., 1987. Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis inXanthomonas campestris. Journal of Bacteriology 169: 2854–2861.
Hötte, B., Ruth-Arnold, I., Pühler, A. & Simon, R., 1990. Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production inXanthomonas campestris pv.campestris. Journal of Bacteriology 172: 2804–2807.
Ielpi, L., Couso, R. & Dankert, M., 1981a. Lipid linked intermediates in the biosynthesis of xanthan gum. FEBS Letters 130: 253–256.
Ielpi, L., Couso, R. & Dankert, M.A., 1981b. Xanthan gum biosynthesis pyruvic acid acetal residues are transferred from phosphoenol pyruvate to the pentasaccharide-P-P-lipid. Biochemical Biophysical Research Communications 102: 1400–1408.
Kamoun, S., & Kado, C.I., 1990. A plant inducible gene ofXanthomonas campestris pv. campestris encodes an exocellular component required for growth in the host and hypersensitivity on non hosts. Journal of Bacteriology 172: 5165–5172.
Klement, Z., 1982. Hypersensitivity. In: Mount, M.S. & Lacy, G.H. (Eds), Phytopathogenic prokaryotes, Vol. 2. Academic Press, New York. p. 149–177.
Lamb, C., Lawton, M., Dron, M. & Dixon, R., 1989. Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell 56: 215–224.
Leyns, F., De Cleene, M., Swings, J.G. & De Ley, F., 1984. The host range of the genusXanthomonas. Botanical Review 50: 308–356.
Lindgren, P.B., Peet, R.C. & Panopoulos, N.J., 1986. Gene cluster ofPseudomonas syringae pv. ‘phaseolicola’ controls pathogenicity on bean plants and hypersensitivity on non host plants. Journal of Bacteriology 168: 512–522.
Liu, Y.N., Tang, J.L., Clarke, B.R., Dow, J.M. & Daniels, M.J., 1990. A multipurpose broad host range cloning vector and its use to characterize an extracellular protease gene ofXanthomonas campestris pv.campestris. Molecular and General Genetics 220: 433–440.
Meyerowitz, E.M., 1989.Arabidopsis, a useful weed. Cell 56: 263–269.
Osbourn, A.E., Barber, C.E. & Daniels, M.J., 1987. Identification of plant-induced genes of the bacterial pathogenXanthomonas campestris pathovarcampestris using a promoter-probe plasmid. The EMBO Journal 6: 23–28.
Osbourn, A.E., Clarke, B.R., Stevens, B.J.H. & Daniels, M.J., 1990a. Use of oligonucleotide probes to identify members of two-component regulatory systems inXanthomonas campestris pathovarcampestris. Molecular and General Genetics 222: 145–151.
Osbourn, A.E., Clarke, B.R. & Daniels, M.J., 1990b. Identification and DNA sequence of a pathogenicity gene ofXanthomonas campestris pv.campestris. Molecular Plant-Microbe Interactions 3: 280–285.
Pugsley, A.P., D'Enfert, C., Reyss, I. & Kornacker, M.G., 1990. Genetics of extracellular protein secretion by gram negative bacteria. Annual Review of Genetics 24: 67–90.
Simpson, R.B. & Johnson, L.J., 1990.Arabidopsis thaliana as a host forXanthomonas campestris pv.campestris. Molecular Plant-Microbe Interactions 3: 233–237.
Stock, J.B., Ninfa, A.J. & Stock, A.M., 1989. Protein phosphorylation and regulation of adoptive responses in bacteria. Microbiological Reviews 53: 450–490.
Tang, J.L., Gough, C.L., Barber, C.E., Dow, J.M. & Daniels, M.J., 1987. Molecular cloning of protease gene(s) fromXanthomonas campestris pv.campestris: Expression inEscherichia coli and role in pathogenicity. Molecular and General Genetics 210: 443–448.
Tang, J.L., Gough, C.L. & Daniels, M.J., 1990. Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide ofXanthomonas campestris pv.campestris. Molecular and General Genetics 222: 157–160.
Tang, J.L., Liu, Y.N., Barber, C.E., Dow, J.M., Wootton, J.C. & Daniels, M.J., 1991. Genetic and molecular analysis of a cluster ofrpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide inXanthomonas campestris pv.campestris. Molecular and General Genetics 226: 409–417.
Thorne, L., Tansy, L. & Pollock, T.J., 1987. Clustering of mutations blocking synthesis of xanthan gum byXanthomonas campestris. Journal of Bacteriology 169: 3593–3600.
Tsuji, J., Somerville, S.C. & Hammerschmidt, R., 1991. Identification of a gene inArabidopsis thaliana that controls resistance toXanthomonas campestris pv.campestris. Physiological and Molecular Plant Pathology 38: 57–65.
Turner, P., Barber, C. & Daniels, M., 1985. Evidence for clustered pathogenicity genes inXanthomonas campestris pv.campestris. Molecular and General Genetics 199: 338–343.
Vanderslice, R.W., Doherty, D.H., Capage, M.A., Betlach, M.R., Hassler, R.A., Henderson, N.M., Ryan-Graniero, J. & Tecklenburg, M., 1989. Genetic engineering of polysaccharide inXanthomonas campestris. In: Crescenzi, V., Dea, I.C.M. & Stivola, S.S. (Eds), Recent developments in industrial polysaccharides: Biomedical and biotechnological advances. Gordon & Breach Science Publishers, New York, p. 145–156
Whalen, M.C., Innes, R.W., Bent, A.F. & Staskawicz, B.J., 1991. Identification ofPseudomonas syringae pathogens ofArabidopsis and a bacterial locus determining avirulence on bothArabidopsis and soybean. The Plant Cell 3: 49–59.
Williams, P.H., 1980. Black rot, a continuing threat to world crucifers. Plant Disease 64: 736–742.
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Daniels, M.J. Interaction of Xanthomonas with crucifers. Netherlands Journal of Plant Pathology 98 (Suppl 2), 147–155 (1992). https://doi.org/10.1007/BF01974481
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DOI: https://doi.org/10.1007/BF01974481