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Effects of high doses of testosterone propionate and testosterone enanthate on rat seminiferous tubules — a stereological and cytological study

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Abstract

The effects of exogenous testosterone on various testicular variables has become of increasing significance because of its potential use in male contraception. For this reason, high doses of two testosterone esters [testosterone propionate (TP) and testosterone enanthate (TE)] were used in a study of their influence on the morphology, length and curvature of the seminiferous tubules of the rat testis, and on cytological smears of the seminiferous tubules epithelium. TP was given for 14 days (3 mg/100 g body weight, i. m.) to assess the acute effects of testosterone on the seminiferous tubules. TE was administered for 60 days (in the same manner as TP) to study possible chronic effects on the rat testis. After TP and TE treatment the seminiferous tubule epithelium showed disorganization and desquamation of spermatogenic cells. In the TP-treated testes the tubules lined with Sertoli cells only were observed. The values for the length and curvature of seminiferous tubules of the TP- and TE-treated rats were significantly reduced (p<0.001). All these changes were observed earlier in the TP-treated than in the TE-treated animals. In cytological smears of the testis of the TP- and TE-treated rats an increase of vacuoles and residual bodies in Sertoli cell cytoplasm was noted. In addition, a reduction of spermatogenic cells, particularly sperms, was manifest in the smears after treatment. Large groups of Sertoli cells were seen in the smears from these testes.

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References

  • Averback P (1983) Testis: seminiferous tubule hypercurvature syndrome. In: Baak JPA, Oort J (eds) A manual of morphometry in diagnostic pathology. Springer, Berlin, pp 115–123

    Google Scholar 

  • Averback P, Wight DGD (1979a) Seminiferous tubules hypercurvature: a newly recognized common syndrome of human male infertility. Lancet I: 181–183

    Article  Google Scholar 

  • Averback P, Wight DGD (1979b) A new cause for male sterility? Lancet I: 384–385

    Google Scholar 

  • Awoniyi CA, Sprando RL, Santulli R, Chandrashekar V, Ewing LL, Zirkin BR (1990) Restoration of spermatogenesis by exogenously administered testosterone in rats made azoospermic by hypophysectomy or withdrawal of luteinizing hormone alone. Endocrinology 127: 177–184

    PubMed  Google Scholar 

  • Banek Lj (1986) Curvature of the seminiferous tubules in infertile human testes. Rad Med Fak 27: 47–52

    Google Scholar 

  • Banek Lj, Posinovec J (1986) Length of the seminiferous tubules in damaged testes. Liječ Vj 108: 170–173

    Google Scholar 

  • Bansal MR, Davies AG (1986) Effect of testosterone oenanthate on spermatogenesis and serum testosterone concentrations in adult mice. J Reprod Fertil 78: 219–224

    PubMed  Google Scholar 

  • Bardin CW (1989) Pituitary-testicular axis. In: Yen SSC, Jaffe RB (eds) Reproductive endocrinology; physiology, pathophysiology and clinical management, 2nd edition. Saunders Company, Philadelphia, pp 177–199

    Google Scholar 

  • Barham SS, Berlin JD (1974) Fine structure and cytochemistry of testicular cells in men treated with testosterone propionate. Cell Tissue Res 148: 159–182

    Article  PubMed  Google Scholar 

  • Berndtson WE, Desjardins C, Ewing LL (1974) Inhibition and maintenance of spermatogenesis in rats implanted with polydimethylsiloxane capsules containing various androgens. J Endocrinol 62: 125–135

    PubMed  Google Scholar 

  • Boccabella AV (1963) Reinitiation and restoration of spermatogenesis with testosterone propionate and other hormones after a long-term post-hypophysectomy regression period. Endocrinology 72: 787–798

    Google Scholar 

  • Conway AJ, Boylan LM, Howe C, Ross G, Handelsman DJ (1988) Randomized clinical trial of testosterone replacement therapy in hypogonadal men. Int J Androl 11: 247–264

    PubMed  Google Scholar 

  • Črepinko I, Posinovec J, Škrabalo Z (1973) Cyto-histological comparison in sterile human testes. Acta Med Iug 27: 449–454

    Google Scholar 

  • Cunningham GR, Huckins C (1979) Persistence of complete spermatogenesis in the presence of low testicular concentrations of testosterone. Endocrinology 105: 177–186

    PubMed  Google Scholar 

  • Desjardins C, Ewing LL, Irby DC (1973) Response of the rabbit seminiferous epithelium to testosterone administrated via polydimethylsiloxane capsules. Endocrinology 93: 450–460

    PubMed  Google Scholar 

  • van Dop PA, Scholtmeyer RJ, Kurver PHJ, Baak JPA, Oort J, Stolte LA (1980) A quantitative structural model of the testis of fertile males with normal sperm counts. Int J Androl 3: 153–169

    PubMed  Google Scholar 

  • Dym M, Fawcett DW (1970) The blood-testis barrier in the rat and physiological compartmentation of the seminiferous epithelium. Biol Reprod 3: 308–326

    PubMed  Google Scholar 

  • Elias H, Hyde DM (1980) Elementary stereology. Am J Anat 159: 411–446

    Article  Google Scholar 

  • Flickinger CJ (1978) Effects of testosterone enanthate on the structure of the male reproductive tract of the rat. Anat Rec 192: 555–584

    Article  PubMed  Google Scholar 

  • Fujioka M, Shinohara Y, Baba S, Irie M, Inoue K (1987) Acute suppression of endogenous testosterone levels by exogenous testosterone in normal men. Life Sci 41: 945–949

    Article  PubMed  Google Scholar 

  • Goldberg RB (1984) Germ cell survival, differentiation, and epididymal transit kinetics in mouse testis subjected to high in vivo levels of testosterone enanthate. Cell Tissue Res 237: 337–342

    Article  PubMed  Google Scholar 

  • Griswold MD (1988) Protein secretions of Sertoli cells. Int Rev Cytol 110: 133–156

    PubMed  Google Scholar 

  • Gunsalus GL, Musto NA, Bardin CN (1978) Immunoassay of androgen binding protein in blood: a new approach for study of the seminiferous tubule. Science 2000: 65–66

    Google Scholar 

  • Infante-Perez V, Bardin CW, Gunsalus GL, Musto NA, Rich KA, Mather JP (1986) Differential regulation of testicular transfferrin and androgen-binding protein secretion in primary cultures of rat Sertoli cells. Endocrinology 118: 383–392

    PubMed  Google Scholar 

  • Kališnik M (1985) Temelji stereologije. Acta Stereol 4: 1–148

    Google Scholar 

  • Kurohmaru M, Hikim APS, Mayerhofer A, Bartke A, Russell LD (1990) Golden hamster myoid cells during active and inactive states of spermiogenesis: correlation of testosterone levels with structure. Am J Anat 188: 319–327

    Article  PubMed  Google Scholar 

  • Lamb DJ, Tsai YH, Steinberger A, Sanborn BM (1981) Sertoli cell nuclear transcriptional activity: stimulation by follicle-stimulating hormone and testosterone in vitro. Endocrinology 108: 1020–1026

    PubMed  Google Scholar 

  • Lanz T, Neuhäuser G (1963) Metrische Untersuchungen an den Tubuli contorti des menschlichen Hodens. Z Anat Entwickl Gesch 123: 462–489

    Article  Google Scholar 

  • Larmont MA, Faed MJW, Baxby K (1981) Comparative studies of spermatogenesis in fertile and subfertile men. J Clin Pathol 34: 145–150

    PubMed  Google Scholar 

  • Lennox B (1979) A new cause for male sterility? Lancet I: 384–385

    Article  Google Scholar 

  • Lennox B, Ahmad KN (1971) The total length of tubules in the human testes. J Anat 107: 191

    Google Scholar 

  • Lennox B, Ahmad KN, Mack WS (1970a) A method for determining the relative total length of the tubules in the testis. J Pathol 102: 229–238

    Article  PubMed  Google Scholar 

  • Lennox B, Ahmad KN, Mack WS (1970b) The total length of tubules in normal and atrophic testes. J Pathol 100: 3–4

    Google Scholar 

  • Ludwig DJ (1950) The effects of androgen on spermatogenesis. Endocrinology 46: 453–481

    PubMed  Google Scholar 

  • Matsumoto AM (1988) Is high dosage testosterone an effective male contraceptive agent? Fertil Steril 50: 324–328

    PubMed  Google Scholar 

  • Matsumoto AM (1990) Effects of chronic testosterone administration in normal men: safety and efficacy of high dosage testosterone and parallel dose-dependent suppression of luteinizing hormone, follicle-stimulating hormone and sperm production. J Clin Endocrinol Metab 70: 282–287

    PubMed  Google Scholar 

  • Papić Z, Katona G, Škrabalo Z (1988) The cytologic identification and quantification of testicular cell subtypes; reproducibility and relation to histologic findings in the diagnosis of male fertility. Acta Cytol 32: 697–706

    PubMed  Google Scholar 

  • Parvinen M, Huhtaniemi I (1990) Testosterone micromilieu in staged rat seminiferous tubules. J Steroid Biochem 36: 377–381

    Article  PubMed  Google Scholar 

  • Pelliniemi LJ, Dym M, Gunsalus GL, Musto NA, Bardin CW, Fawcett DW (1981) Immunocytochemical localization of androgen-binding protein in the male rat reproductive tract. Endocrinology 108: 925–931

    PubMed  Google Scholar 

  • Russel LD, Clermont Y (1976) Degeneration of germ cells in normal, hypophysectomized and hormone treated rats. Anat Rec 187: 347–366

    Article  Google Scholar 

  • Sanborn BM, Steinberger A, Tcholakian RK, Steinberger E (1977) Direct measurement of androgen receptors in cultured Sertoli cells. Steroids 29: 493–502

    Article  PubMed  Google Scholar 

  • Santulli R, Sprando RL, Awoniyi CA, Ewing LL, Zirkin BR (1990) To what extent can spermatogenesis be maintained in the hypophysectomized adult rat testis with exogenously administered testosterone? Endocrinology 126: 95–101

    PubMed  Google Scholar 

  • Schanbacher BD (1980) Dose dependent inhibition of spermatogenesis in mature rams with exogenous testosterone. Int J Androl 3: 563–573

    PubMed  Google Scholar 

  • Schenck U, Schill W (1988) Cytology of the human seminiferous epithelium. Acta Cytol 32: 689–696

    PubMed  Google Scholar 

  • Sharpe RM (1987) Testosterone and spermatogenesis. J Endocrinol 113: 1

    PubMed  Google Scholar 

  • Sharpe RM, Donachie K, Cooper I (1988) Re-evaluation of the intratesticular level of testosterone required for quantitative maintenance of spermatogenesis in the rat. J Endocrinol 117: 19–26

    PubMed  Google Scholar 

  • Sharpe RM, Maddocks S, Kerr JB (1990) Cell-cell interactions in the control of spermatogenesis as studied using Leydig cell destruction and testosterone replacement. Am J Anat 188: 3–20

    Article  PubMed  Google Scholar 

  • Steinberger E (1971) Hormonal control of spermatogenesis. Physiol Rev 51: 1–22

    Google Scholar 

  • Steinberger E, Duckett GE (1965) Effect of estrogen or testosterone on initiation and maintenance of spermatogenesis in the rat. Endocrinology 76: 1184–1189

    PubMed  Google Scholar 

  • Sun Y-T, Wreford NG, Robertson DM, de Kretser DM (1990) Quantitative cytological studies of spermatogenesis in intact and hypophysectomized rats; identification of androgen-dependent stages. Endocrinology 1215–1223

  • Swinnen K, Cailleau J, Heyns W, Verhoeven G (1990) Prostatic testicular peritubular cells produce similar paracrine mediators of androgen action. Endocrinology 126: 142–150

    PubMed  Google Scholar 

  • Tâhkâ KM (1989) Local control mechanisms in the testis. Int J Dev Biol 33: 141–148

    PubMed  Google Scholar 

  • Tcholakian RK, Steinberger A (1979) In vitro metabolism of testosterone by cultured Sertoli cells and the effect of FSH. Steroids 33: 495–513

    Article  PubMed  Google Scholar 

  • Tcholakian RK, Steinberger A (1980) In vitro metabolism of testosterone by Sertoli cells and interstitial cells and the effects of FSH. In: Steinberger A, Steinberger E (eds) Testicular development, structure and function. Raven Press, New York, pp 177–183

    Google Scholar 

  • Tsai YH, Sanborn BM, Steinberger A, Steinberger E (1977) The interaction of testicular androgen-receptor complex with rat germ cell and Sertoli cell chromatin. Biochem Biophys Res Comm 75: 366–372

    Article  PubMed  Google Scholar 

  • Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30: 23–31

    PubMed  Google Scholar 

  • Wilson JD, Griffin JE (1980) The use and misuse of androgens. Metabolism 29: 1278–1295

    Article  PubMed  Google Scholar 

  • World Health Organization Task Force on Methods for the Regulation of Male Fertility (1990) Contraceptive efficacy of testosterone-induced azoospermia in normal men. Lancet 336: 955–959

    Google Scholar 

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Authors and Affiliations

  1. Institute of Histology and Embryology, Medical Faculty University of Zagreb, Šalata 3, PO Box 166, 41000, Zagreb, Croatia

    Davor Ježek, Ljerka Šimunić-Banek & Ružica Pezerović-Panijan

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  1. Davor Ježek
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  2. Ljerka Šimunić-Banek
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  3. Ružica Pezerović-Panijan
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The study was supported by a Grant for Scientific Research No. 3-01-041 from the Ministry of Science, Technology and Informatics of the Republic of Croatia

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Ježek, D., Šimunić-Banek, L. & Pezerović-Panijan, R. Effects of high doses of testosterone propionate and testosterone enanthate on rat seminiferous tubules — a stereological and cytological study. Arch Toxicol 67, 131–140 (1993). https://doi.org/10.1007/BF01973684

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  • DOI: https://doi.org/10.1007/BF01973684

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