Abstract
Amoscanate, a substance which damages photoreceptors, was administered orally to Wistar rats in doses of 10, 40, and 125 mg/kg body weight once daily for 3 or 10 days. At both times electroretinographic, ophthalmological, and histopathological examinations of the retina were carried out to compare the sensitivity of conventional methods and to test electroretinography (ERG) for suitability for use in toxicity studies. Time-dependent and dose-dependent effects were found by electroretinography and light microscopy. However, signs of retinal changes appeared earlier and more distinctly in the electroretinogram. Ophthalmological fundus examination in albino rats yielded no characteristic correlate. In conclusion, electroretinography constitutes a valuable supplement to histopathology and is suitable for use in toxicity studies.
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References
Ahmed B, El-Wafai S, Bana L (1989) Effect of elevation of body temperature on the electroretinograms of the rat. Int J Hyperthermia 5: 675–682
Armington JC (1974) The electroretinogram. Academic Press Inc., New York London, pp 177, 380–427
Butler WH, Ford GP, Newberne JW (1987) A study of the effects of vigabatrin on the central nervous system and retina of Sprague-Dawley and Lister-Hooded rats. Toxicol Pathol 15: 143–148
Chang LW, Dyer RS (1983) Trimethyltin induced pathology in sensory neurons. Neurobehav Toxicol Teratol 5: 673–696
Dowling E, Sidman RL (1962) Inherited retinal dystrophy in rat. J Cell Biol 14: 73–109
El Azazi M, Wachtmeister L (1990) The postnatal development of the oscillatory potentials of the electroretinogram: I. Basic characteristics. Acta Ophthalmol 68: 401–409
Federspiel-Eisenring E, Reme C, Pfeilschifter J (1988) Lithium-Schaeden in der Ratten-Netzhaut — Verstaerkung durch Licht. Klin Monatsbl Augenheilkd 192: 134–140
Foerster MH (1986) Klinische Aspekte des DC-Elektrorentinogramms. Habilitationsschrift, Universitaetsklinikum Essen, pp 39–42
Fox DA, Chu LWF (1988) Rods are selectively altered by lead: II. Ultrastructure and quantitative histology. Exp Eye Res 46: 613–625
Gilad GM, Gilad VH (1989) Treatment with polyamines can prevent monosodium glutamate neurotoxicity in the rat retina. Life Sci 44: 1963–1969
Hebel R, Stromberg MW (1986) Anatomy and embryology of the laboratory rat. BioMed Verlag, Wörthsee, p 219
Imai H, Miyata M, Uga S, Ishikawa S (1983) Retinal degeneration in rats exposed to an organophosphate pesticide (fenthion). Environ Res 30: 453–465
International Standardization Committee (1989) Standard for clinical electroretinography. Arch Ophthalmol 107: 816–819
Ivanina TA, Sakina NL, Lebedeva MN, Borovyagin VL (1989) A study of the mechanisms of chloroquine retinopathy. Ophthalmic Res 21: 216–220
Korte GE, Bellhorn RW, Burns MS (1984) Urethane-induced rat retinopathy. Invest Ophthalmol Vis Sci 25: 1027–1034
Krinke G, Graepel P, Krueger L, Thomann P (1983) Early effects of high-dosed absorbable amoscanate on rat brain. Toxicol Lett 19: 261–266
Kuhn FJ, Weisse I (1990): The ERG as a complementary diagnostic tool in ophthalmoscopy in albino rats. Lens Eye Toxic Res 7: 667–676
Niemeyer G (1979) Information conveyed by electroretinography. Albert v Graefe Arch Klin Exp Ophthalmol 211: 129–137
Noell WK, Walker VS, Kang BS, Berman S (1966) Retinal damage by light in rats. Invest Ophthalmol 5: 450–473
Penn JS, Thum LA (1989) The rat as an animal model for retinopathy of prematurity. In: Lavail MN, Anderson RE, Hollyfield JG (eds) Progress in clinical and biological research — inherited and environmentally induced retinal degenerations, vol. 314. Liss Inc., New York, pp 625–642
Ruchoux MM, Droy-Lafaix TD, Bakri F, Guillemain J, Ruchoux P, Autret A (1990) Cicletanine and hypertensive retinopathy. Ophthalmic Res 22: 318–329
Sandberg MA, Pawlyk BS, Crane WG, Berson EL (1988) Diurnal rhythm in the electroretinogram of the Royal College of Surgeons (RCS) pigmented rat. Exp Eye Res 46: 929–936
Schaeppi U, Krinke G, Fitzgerald RE, Ziel R (1987) Retinotoxicity of amoscanate in the albino rat. Concepts Toxicol 4: 179–182
Schmidt JGH (1969) Revival time of the b-wave of the electroretinogram following retinal and choroidal ischemia as related to various body and eye temperatures. VII ISCERG Symposium, Istambul
Trejo LJ, Rand MN, Cicerone CM (1989) Consensual pupillary light reflex in the pigmented rat. Vision Res 29: 303–307
Winkler BS (1972) The electroretinogram of the isolated rat retina. Vision Res 12: 1183–1198
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Maertins, T., Kroetlinger, F., Sander, E. et al. Electroretinographic assessment of early retinopathy in rats. Arch Toxicol 67, 120–125 (1993). https://doi.org/10.1007/BF01973682
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DOI: https://doi.org/10.1007/BF01973682