Abstract
A commercial enzyme immunoassay (Platelia-Toxo IgA) and an immunoblot technique were compared with regard to their ability to detect IgA antibodies to the major surface protein P30(SAG1) ofToxoplasma gondii in 105 serum samples from patients with suspected or proven acquired toxoplasmosis. Comparison of the IgA-EIA with the immunoblot technique showed a concordance of 81.0 %, with a sensitivity of 92.6 % and a specificity of 78.4 %. Due to its high sensitivity the IgA-EIA might detect IgA antibodies againstToxoplasma gondii at an early stage of infection, although excessive sensitivity could lead to detection of IgA antibodies for an extended period of time following the onset of infection.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Sabin AB, Feldman HA Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science 1949, 108: 660–663.
Walton BC, Benchoff BM, Brooks WH Comparison of indirect fluorescent antibody test and methylene blue dye test for detection of antibodies toToxoplasma gondii. American Journal of Tropical Medicine and Hygiene 1966, 15: 149–152.
Warren J, Sabin AB The complement fixation reaction in toxoplasmic infection. Proceedings of the Society of Experimental Biology and Medicine 1942, 51: 11–14.
Cesbron JY, Capron A, Ovlaque G, Santoro F Use of a monoclonal antibody in a double-sandwich ELISA for detection of IgM antibodies toToxoplasma gondii major surface protein (P30). Journal of Immunological Methods 1985, 83: 151–158.
Remington JS, Desmonts G Toxoplasmosis. In: Remington JS, Klein JO (ed): Infectious diseases of the fetus and newborn infant. Saunders, Philadelphia, 1990, p. 89–195.
Brooks RG, McCabe RE, Remington JS Role of serology in the diagnosis of toxoplasmic lymphadenopathy. Reviews of Infectious Diseases 1987, 9: 1055–1062.
Stepick-Biek P, Thulliez P, Araujo FG, Remington JS IgA antibodies for diagnosis of acute congenital and acquired toxoplasmosis. Journal of Infectious Diseases 1990, 162: 270–273.
Huskinson J, Thulliez P, Remington JS Toxoplasma antigens recognized by human immunoglobulin A antibodies. Journal of Clinical Microbiology 1990, 28: 2632–2636.
Decoster A, Darcy F, Caron A, Capron A IgA antibodies against P30 as markers of congenital and acute toxoplasmosis. Lancet 1988, ii: 1104–1107.
Partanen P, Turunen HJ, Paasivuo RTA, Leinikki PO Immunoblot analysis ofToxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection. Journal of Clinical Microbiology 1984, 20: 133–135.
Bessières MH, Roques C, Berrebi A, Barre V, Cazaux M, Séguéla JP IgA antibody response during acquired and congenital toxoplasmosis. Journal of Clinical Pathology 1992, 45: 605–608.
Groß U, Roos T, Appoldt D, Heesemann J Improved serological diagnosis ofToxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique. Journal of Clinical Microbiology 1992, 30: 1436–1441.
Groß U, Müller J, Roos T, Schrod L, Heesemann J Possible reasons for failure of conventional tests for diagnosis of fatal congenital toxoplasmosis: report of a case diagnosed by PCR and immunoblot. Infection 1992, 10: 149–152.
Decoster A, Slizewitz B, Simon J, Bazin C, Darcy F, Vittu G, Boulanger C, Champeau Y, Demory JL, Duhamel M, Capron A Platelia-Toxo IgA, a new kit for early diagnosis of congenital toxoplasmosis by detection of anti-P30 immunoglobulin A antibodies. Journal of Clinical Microbiology 1991, 29: 2291–2295.
Groß U, Roggenkamp A, Janitschke K, Heesemann J Improved sensitivity of the polymerase chain reaction for detection ofToxoplasma gondii in biological and human clinical specimen. European Journal of Clinical Microbiology & Infectious Diseases 1992, 11: 33–39.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Groß, U., Bohne, W., Schröder, J. et al. Comparison of a commercial enzyme immunoassay and an immunoblot technique for detection of immunoglobulin A antibodies toToxoplasma gondii . Eur. J. Clin. Microbiol. Infect. Dis. 12, 636–639 (1993). https://doi.org/10.1007/BF01973647
Issue Date:
DOI: https://doi.org/10.1007/BF01973647