Skip to main content
SpringerLink
Log in

The influence of carbaryl on the uptake of (3H)-noradrenaline (3H)-NA by rat hypothalamic slices

  • Short Communication
  • Published:
Archives of Toxicology Aims and scope Submit manuscript

Abstract

The uptake of (3H)-NA by rat hypothalamic slices was investigated following carbaryl poisoning. The insecticide was administered to rats orally in a single dose of 50% LD50 and of 20% LD50, and in repeated dosing for 14 days (5% LD50 daily). The kinetics of (3H)-NA uptake was defined by the modified Lineweaver-Burk plots (Rmax was used instead of Vmax and Km was replaced with t05 max). The results indicated that in all the experiments carbaryl elevated the (3H)-NA uptake by hypothalamic slices in a dosage dependent fashion (insignificantly at the 20% LD50 dose level,p >0.05) as evidenced by the increased Rmax values. However, carbaryl simultaneously decreased the kinetics of the process in the hypothalamus since t0.5max values were enhanced (insignificantly at the 5% LD50 dose level,p >0.05). Carbaryl produced a dosedependent increase of t0.5max values. The changes in the (3H)-NA uptake may be related to the increase of NA level in the hypothalamus following exposure to carbaryl observed by other authors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Branch RA, Jacqz E (1986) Subacute neurotoxicity following long-term exposure to carbaryl. Am J Med 4: 741–745

    Article  Google Scholar 

  • Carmichael FJ, Israel Y (1973) In vitro inhibitory effect of narcotic analgesics other psychotropic drugs on the active uptake of norepinephrine in mouse brain slices. J Pharmacol Exp Ther 186: 253–260

    PubMed  Google Scholar 

  • Deśi I, Gonczi L, Simon G, Farkas I, Kneffel Z (1974) Neurotoxicologic studies of two carbamate pesticides in subacute animal experiments. Toxicol Appl Pharmacol 27: 465–476

    Article  PubMed  Google Scholar 

  • Dickoff DJ, Gerber O, Turowsky Z (1987) Delayed neurotoxicity after ingestion of carbamate pesticide. Neurology 37: 1229–1231

    PubMed  Google Scholar 

  • Glowinski J, Iversen LL (1966) Regional studies of catecholamines in the rat brain. 1. The disposition of (3H)-norepinephrine, (3H)dopamine and (3H)-dopa in various regions of the brain. J Neurochem 13: 655–669

    PubMed  Google Scholar 

  • Hassan A (1971) Pharmacological effects of Carbaryl. I. The effect of carbaryl on the synthesis and degradation of catecholamines in the rat. Biochem Pharmacol 20: 2299–2308

    Article  PubMed  Google Scholar 

  • Hassan A, Santolucito JA (1971) Pharmacological effects of Carbaryl. II. Modification of serotonin metabolism in the rat brain. Experientia 27: 287–288

    PubMed  Google Scholar 

  • Kontek M, Marcinkowska B, Pietraszek Z (1973) Badania encefalograficzne u pracowników rolnych eksponowanych na pestycydy z grupy zwiazków fosforoorganicznych i karbaminianów. Pol Tyg Lek 28: 1879–1881

    PubMed  Google Scholar 

  • Mount ME, Dayton AD, Oehme FW (1981) Carbaryl residues in tissues and cholinesterase activities in brain and blood of rats receiving carbaryl. Toxicol Appl Pharmacol 58: 282–296

    Article  PubMed  Google Scholar 

  • Ray SK, Haque SJ, Poddar MK (1984) Effects of carbaryl on catecholamines in brain regions. Indian J Exp Biol 22: 141–144

    PubMed  Google Scholar 

  • Ray SK, Poddar MK (1985) Effect of pentylenetetrazol on carbaryl induced changes in striatal catecholamines. Biochem Pharmacol 34: 553–557

    Article  PubMed  Google Scholar 

  • Santolucito JA (1970) Comparison of chronic and acute low level exposure effects of Carbaryl on the EEG of squirrel monkeys. Ind Med Surg 39: 315–321

    Google Scholar 

  • Santolucito JA, Morrison G (1971) EEG of rhesus monkeys following prolonged low-level feeding of pesticides. Toxicol Appl Pharmacol 19: 147–154

    Article  PubMed  Google Scholar 

  • Smalley HE (1970) Diagnosis and treatment of carbaryl poisoning in the swine. J Am Vet Med Assoc 156: 339–344

    PubMed  Google Scholar 

  • Smalley HE, O'Hara PJ, Bridges ChH, Radeleff RD (1968) The effect of chronic Carbaryl administration on the neuromuscular system of swine. Toxicol Appl Pharmacol 14: 409–419

    Article  Google Scholar 

  • Snyder SH, Green AJ, Handley ED (1968) Kinetics of (3H)-norepinephrine accumulation into slices from different regions of the rat brain. J Pharmacol Exp Ther 164: 90–102

    PubMed  Google Scholar 

  • Vandekar M, Plestina R, Wilhelm K (1971) Toxicity of carbamates for mammals. Bull WHO 44: 241–249

    PubMed  Google Scholar 

  • Weil CS (1952) Tables for convenient calculation of median-effective dose (LD50 or ED50) and instruction in their use. Biometrics 8: 249–263

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Toxicology, Medical Academy Warsaw, 1 Banacha Street, PL-02-097, Warsaw, Poland

    Joanna Jablońska & Jacek Brzeziński

Authors
  1. Joanna Jablońska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jacek Brzeziński
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jablońska, J., Brzeziński, J. The influence of carbaryl on the uptake of (3H)-noradrenaline (3H)-NA by rat hypothalamic slices. Arch Toxicol 64, 417–419 (1990). https://doi.org/10.1007/BF01973467

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01973467

Key words

Search

Navigation