Comparative uptake, vascular transport, and cellular internalization of aflatoxin-B1 and benzo(a)pyrene
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Studies of the uptake of benzo(a)pyrene (BaP) and aflatoxin-B1 (AFB1) after gastric instillation showed that BaP was absorbed via the intestinal lymphatic drainage and transported to the vascular circulation sequestered within lipoproteins in thoracic duct lymph, while AFB1 was absorbed with water soluble compounds into the gastrointestinal venous drainage and was not transported in association with lipoproteins. BaP was taken up into plasma lipoproteins over a broad concentration range, while AFB1 was not sequestered within lipoproteins over the same concentration range. Low density lipoproteins (LDL) facilitated BaP uptake into fibroblasts and impeded BaP uptake into hepatocytes. High density lipoproteins (HDL) facilitated BaP uptake into hepatocytes and impeded BaP uptake into fibroblasts. The uptake of AFB1 into either fibroblasts or hepatocytes was not affected by lipoproteins.
Key wordsAflatoxin-B1 Benzo(a)pyrene Vascular transport Lipoproteins Cellular internalization
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- Brown MS, Anderson RGW, Goldstein JL (1983) Recycling receptors: the round trip itinerary of migrant membrane proteins. Cell 32: 662–667Google Scholar
- Dashek WV, Barker SM, Statkiewicz WR, Shanks ET (1982) Histochemical analysis of liver cells from short term, aflatoxin-dosed and nondosedCoturnix coturnix japonica. 1. Aflatoxin-sensitive quail. Poultry Science 62: 2347–2359Google Scholar
- Keenan RW, Kruczek ME, Fischer JB (1977) The binding of [3H]-dolichol by plasma high density lipoproteins. Biochim Biophys Acta 486: 1–9Google Scholar
- Kocan RM, Chi EY, Eriksen N, Benditt EP, Landolt ML (1983) Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro. Environ Mutagen 5: 647–656Google Scholar
- Kotin P, Falk HL, Busser R (1959) Distribution, retention, and elimination of [14C]-3,4-benzopyrene after administration to mice and rats. J Natl Cancer Inst 23: 541–555Google Scholar
- McQueen CA, Kreiser DM, Williams GM (1983) The hepatocyte primary culture/DNA repair assay using mouse or hamster hepatocytes. Environ Mutagen 5: 1–8Google Scholar
- Plant AL, Benson DM, Smith LC (1985) Cellular uptake and intracellular localization of benzo(a)pyrene by digital fluorescence microscopy. J Cell Biol 100: 1925–1308Google Scholar
- Salocks CB, Hsieh DP, Byard JL (1981) Butylated hydroxytoluene pretreatment protects against cytotoxicity and reduces covalent binding of aflatoxin B1 in primary hepatocyte cultures. Pharmacol Appl Toxicol 59: 331–345Google Scholar
- Weisberger JH, Grantham PH, Weisberger EK (1969) The transport of chemical carcinogens by blood. In: Bergmann ED, Pullman B (eds) The Jerusalem Symposium on Quantum Chemistry and Biochemistry, Physico-Chemical Mechanisms of Carcinogenesis. Vol. 1 pp 262–283Google Scholar
- Yoo JS, Norman JO, Busbee DL (1984) Uptake of benzo[a]pyrene into human lipoproteins: triglyceride content as the determining factor Exp Biol Med 177: 434–440Google Scholar
- Ziprin RL, Ellissalde MH, Clark DE, Wilson RD (1980) Absorption of polychlorinated biphenyl by the ovine lymphatic system. Vet Hum Toxicol 22 (5): 304–308Google Scholar