Abstract
Studies of the uptake of benzo(a)pyrene (BaP) and aflatoxin-B1 (AFB1) after gastric instillation showed that BaP was absorbed via the intestinal lymphatic drainage and transported to the vascular circulation sequestered within lipoproteins in thoracic duct lymph, while AFB1 was absorbed with water soluble compounds into the gastrointestinal venous drainage and was not transported in association with lipoproteins. BaP was taken up into plasma lipoproteins over a broad concentration range, while AFB1 was not sequestered within lipoproteins over the same concentration range. Low density lipoproteins (LDL) facilitated BaP uptake into fibroblasts and impeded BaP uptake into hepatocytes. High density lipoproteins (HDL) facilitated BaP uptake into hepatocytes and impeded BaP uptake into fibroblasts. The uptake of AFB1 into either fibroblasts or hepatocytes was not affected by lipoproteins.
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References
Alam SS, Solen KA, Layman DL, Riddle MD, Connor WE (1980) The mass uptake of cholesterol ester from low density lipoproteins by cultured smooth muscle and adventitial cells of human aortas. Circ Res 47: 374–383
Brown MS, Anderson RGW, Goldstein JL (1983) Recycling receptors: the round trip itinerary of migrant membrane proteins. Cell 32: 662–667
Busbee DL, Payne DM, Jasheway DW, Carlisle S, Lacko AG (1981) Separation and detection of lipoproteins in human serum by use of size-exclusion liquid chromatography: a preliminary report. Clin Chem 27: 2052–2059
Busbee DL, Rankin PW, Payne DM, Jasheway DW (1982) Binding of benzo[a]pyrene and intracellular transport of a bound electrophilic benzo[a]pyrene metabolite by lipoproteins. Carcinogenesis 3 (10): 1107–1112
Busbee DL, Joe CO, Ranklin PW, Ziprin RL, Wilson RD (1984) Benzo[a]pyrene uptake by lymph: a possible transport mode for immunosuppressive chemicals. J Toxicol Environ Health 13: 43–51
Chalmer JG (1955) The adsorption of 3,4-benzopyrene and its fluorescent metabolites on serum proteins. Br J Cancer 9: 320–326
Chen TC, Bradley WA, Gotto AM Jr, Morrisett JD (1979) Binding of the chemical carcinogenp-dimethylamino-azobenzene, by plasma low density lipoproteins. FEBS Lett 104: 236–240
Coulombe RA Jr, Wilson DW, Hsieh DPH (1984) Metabolism, DNA binding, and cytotoxicity of aflatoxin B1 in tracheal explants from Syrian hamster. Toxicology 32: 117–130
Dashek WV, Barker SM, Statkiewicz WR, Shanks ET (1982) Histochemical analysis of liver cells from short term, aflatoxin-dosed and nondosedCoturnix coturnix japonica. 1. Aflatoxin-sensitive quail. Poultry Science 62: 2347–2359
Franklin RB, Elcombe CR, Vodicnik MJ, Lechw JJ (1980) Comparative aspects of the disposition and metabolism of xenobiotics in fish and mammals. Fed Proc 39 (13): 3144–3149
Goldstein JL, Brown MS (1974) Binding and degradation of low density lipoproteins by cultured human fibroblasts. J Biol Chem 249: 5153–5162
Goldstein JL, Ho YK, Basu SK, Brown MS (1979) Binding sites on macrophages that mediate uptake and degradation of acetylated low density lipoproteins, producing massive cholesterol deposition. Proc Natl Acad Sci USA 76: 333–337
Grubbs CJ, Moon RC (1973) Transport of orally administered 9, 10-dimethyl-1,2-benzanthracene in the Sprague-Dawley rat. Cancer Res 33: 1785–1789
Hanigan HM, Laishes BA (1984) Toxicity of aflatoxin B1 in rat and mouse hepatocytes in vivo and in vitro. Toxicology 30: 185–193
Irvin TR, Wogan GN (1984) Quantitation of aflatoxin B1 adduction within the ribosomal RNA gene sequences of rat liver DNA. Proc Natl Acad Sci USA 81: 664–668
Kaminski NE, Wells DS, Dauterman WC, Roberts JF, Guthrie FE (1986) Macrophage uptake of a lipoprotein-sequestered toxicant: A potential route of immunotoxicity. Toxicol Appl Pharmacol 82 (3): 474–480
Keenan RW, Kruczek ME, Fischer JB (1977) The binding of [3H]-dolichol by plasma high density lipoproteins. Biochim Biophys Acta 486: 1–9
Kocan RM, Chi EY, Eriksen N, Benditt EP, Landolt ML (1983) Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro. Environ Mutagen 5: 647–656
Kotin P, Falk HL, Busser R (1959) Distribution, retention, and elimination of [14C]-3,4-benzopyrene after administration to mice and rats. J Natl Cancer Inst 23: 541–555
Maliwal BP, Guthrie FE (1981) Interaction of insecticides with human plasma lipoproteins. Chem Biol Interact 35 (2): 177–188
Maliwal BP, Guthrie FE (1982) In vitro uptake and transfer of chlorianted hydrocarbons among human lipoproteins. J Lipid Res 23: 474–479
McQueen CA, Kreiser DM, Williams GM (1983) The hepatocyte primary culture/DNA repair assay using mouse or hamster hepatocytes. Environ Mutagen 5: 1–8
O'Brien K, Moss E, Judah D, Neal G (1983) Metabolic basis of the species difference to aflatoxin B1-induced hepatotoxicity. Biochem Biophys Res Commun 114 (2): 813–821
Plant AL, Benson DM, Smith LC (1985) Cellular uptake and intracellular localization of benzo(a)pyrene by digital fluorescence microscopy. J Cell Biol 100: 1925–1308
Poland A, Glover E, Kende AS (1976) Stereospecific, high affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by hepatic cytosol. J Biol Chem 251: 4936–4945
Remsen J, Shireman R (1981) Effect of low density lipoprotein on the incorporation of benzo(a)pyrene by cultured cells. Cancer Res 41: 3179–3185
Salocks CB, Hsieh DP, Byard JL (1981) Butylated hydroxytoluene pretreatment protects against cytotoxicity and reduces covalent binding of aflatoxin B1 in primary hepatocyte cultures. Pharmacol Appl Toxicol 59: 331–345
Shireman RB, Schneider M (1982) Association of aflatoxin B1 with plasma components in vitro. Toxicol Lett 14: 213–220
Shireman RB, Kilgore LL, Fisher WR (1977) Solubilization of apolipoprotein B and its specific binding by the cellular receptor for low density lipoprotein. Proc Natl Acad Sci USA 74: 5150–5154
Shu HP, Nichols AV (1979) Benzo[a]pyrene uptake by human plasma lipoproteins in vitro. Cancer Res 39: 1224–1230
Wandel M, Norum KR, Berg T, Ose L (1981) Binding, uptake, and degradation of [125I]-labeled high-density lipoproteins in isolated non-parenchymal rat liver cells. Scand J Gastroenterol 16: 1–10
Weisberger JH, Grantham PH, Weisberger EK (1969) The transport of chemical carcinogens by blood. In: Bergmann ED, Pullman B (eds) The Jerusalem Symposium on Quantum Chemistry and Biochemistry, Physico-Chemical Mechanisms of Carcinogenesis. Vol. 1 pp 262–283
Wilson RD, Ziprin RL, Clark DE, Ellissalde MH (1982) Absorption of pentachlorophenol by the ovine lymphatic system: a technical note Vet Hum Toxicol 24 (1): 12–15
Wilson RD, Ziprin RL, Ragsdale S, Busbee DL (1985) Uptake and vascular transport of ingested aflatoxin-B1. Toxicol Lett 29: 169–176
Wong ZA, Hsieh DPH (1980) The comparative metabolism and toxicokinetics of aflatoxin B1 in the monkey, rat, and mouse. Toxicol Appl Pharmacol 55: 115–125
Yoo JS, Norman JO, Busbee DL (1984) Uptake of benzo[a]pyrene into human lipoproteins: triglyceride content as the determining factor Exp Biol Med 177: 434–440
Ziprin RL, Ellissalde MH, Clark DE, Wilson RD (1980) Absorption of polychlorinated biphenyl by the ovine lymphatic system. Vet Hum Toxicol 22 (5): 304–308
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Busbee, D.L., Norman, J.O. & Ziprin, R.L. Comparative uptake, vascular transport, and cellular internalization of aflatoxin-B1 and benzo(a)pyrene. Arch Toxicol 64, 285–290 (1990). https://doi.org/10.1007/BF01972988
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DOI: https://doi.org/10.1007/BF01972988