Summary
Vicia faba (broad bean) root-tip chromosomes were subjected to the SSC-Giemsa and trypsin-Giemsa regimes. Phase-lucent cross-bands seen after fixation/SSC treatment subsequently stain positively with Giemsa. Sequential analysis of the trypsin-Giemsa regime shows, in contrast, that bands are manifest through selective removal of material from interband regions.
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Rowland, R.E. Sequential analysis of Giemsa banded chromosomes in Vicia faba. Experientia 37, 949–951 (1981). https://doi.org/10.1007/BF01971771
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DOI: https://doi.org/10.1007/BF01971771