Abstract
Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains ofStaphylococcus aureus from the local region as controls when molecular typing methods are performed.
Similar content being viewed by others
References
Lyon BR, May JW, Skurray RA Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistantStaphylococcus aureus. Antimicrobial Agents and Chemotherapy 1983, 23: 817–826.
Archer GL, Mayhall CG Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistantStaphylococcus aureus infections. Journal of Clinical Microbiology 1983, 18: 395–399.
Hartstein AI, Morthland VH, Eng S, Archer GL, Schoenknecht FD, Rashad AL Restriction enzyme analysis of plasmid DNA and bacteriophage typing of pairedStaphylococcus aureus blood culture isolates. Journal of Clinical Microbiology 1989, 27: 1874–1879.
Kozarsky PE, Rimland D, Terry PM, Wachsmuth K Plasmid analysis of simultaneous nosocomial out-breaks of methicillin resistantStaphylococcus aureus. Infection Control 1986, 7: 577–581.
Rhinehart E, Shlaes DM, Keyes TF, Serkey J, Kirkley B, Kim C, Currie-McCumber CA, Hall G Nosocomial clonal dissemination of methicillin resistantStaphylococcus aureus: elucidation by plasmid analysis. Archives of Internal Medicine 1987, 147: 521–524.
Takahashi S, Nagano Y Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. Journal of Clinical Microbiology 1984, 20: 608–613.
Zuccarelli AJ, Roy I, Harding GP, Couperus JJ Diversity and stability of restriction enzyme profiles of plasmid DNA from methicillin-resistantStaphylococcus aureus. Journal of Clinical Microbiology 1990, 28: 97–102.
National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. M7-T2. NCCLS, Villanova, PA, 1988.
Nahaie MR, Goodfellow M, Harwood CR A rapid screening procedure for staphylococcal plasmids. Journal of Microbiological Methods 1984, 2: 73–81.
Townsend DE, Ashdown N, Greed LC, Grubb WB Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. Journal of Antimicrobial Chemotherapy 1984, 14: 115–124.
Townsend DE, Ashdown N, Bolton S, Bradley J, Duckworth G, Moorhouse EC, Grubb WB The international spread of methicillin-resistantStaphylococcus aureus. Journal of Hospital Infection 1987, 9: 60–71.
Gelmi M, Foresti I, Ravizzola G, Bonfanti C, Verardi R, Caruso A, Turano A Antibiotic resistances and plasmids inStaphylococcus aureus from Italian hospitals. Journal of Medical Microbiology 1987, 23: 111–118.
Townsend DE, Ashdown N, Momoh M, Grubb WB Distribution of plasmid-borne resistance to nucleic acid binding compounds in methicillin-resistantStaphylococcus aureus. Journal of Antimicrobial Chemotherapy 1985, 15: 417–434.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Doebbeling, B.N., Pfaller, M.A., Hollis, R.J. et al. Restriction endonuclease analysis ofStaphylococcus aureus plasmid DNA from three continents. Eur. J. Clin. Microbiol. Infect. Dis. 11, 4–8 (1992). https://doi.org/10.1007/BF01971263
Issue Date:
DOI: https://doi.org/10.1007/BF01971263