Experientia

, Volume 37, Issue 7, pp 687–689 | Cite as

Irreversible inactivation of yeast glucose-6-P dehydrogenase by penicillin G

  • P. F. Han
  • Y. H. Marian
  • H. W. Matthews
  • J. Johnson
Article

Summary

Yeast glucose-6-P dehydrogenase is irreversibly inactivated by penicillin G. Kinetic data show that 1 molecule of penicillin G reacts with each active unit when the enzyme is inactivated The rate of inactivation increases greatly with increasing pH. This irreversible inactivation by penicillin G is largely prevented by pyridoxal-P, a reversible inactivator of this enzyme. Prior treatment of penicillin G with penicillinase totally abolishes its ability to inactivate the enzyme.

Keywords

Enzyme Penicillin Kinetic Data Active Unit Prior Treatment 

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Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • P. F. Han
    • 1
    • 2
  • Y. H. Marian
    • 1
    • 2
  • H. W. Matthews
    • 1
    • 2
  • J. Johnson
    • 1
    • 2
  1. 1.Department of ChemistryAtlanta UniversityAtlantaUSA
  2. 2.School of PharmacyMercer UniversityAtlantaUSA

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