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Freezing and transplantation of brain tissue in rats

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Summary

Neocortical tissue obtained from rat embryos was frozen and stored at −70°C for 6 h prior to transplantation into the cerebellum of neonatal rats. Growth, differentiation, and integration of this tissue within the host brain was comparable to that obtained from freshly dissected and transplanted tissue. It is suggested that freezing to low temperatures does not adversely effect the viability or transplantability of the neural tissue.

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Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, 47907, West Lafayette, Indiana, USA

    J. D. Houlé & G. D. Das

Authors
  1. J. D. Houlé
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  2. G. D. Das
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Additional information

Supported by N.I.H. research grants Nos NS-08817 and CA-14650.

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Houlé, J.D., Das, G.D. Freezing and transplantation of brain tissue in rats. Experientia 36, 1114–1115 (1980). https://doi.org/10.1007/BF01966001

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