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Contribution of ADP to the amplification of primary platelet aggregation by platelet activating factor (PAF): modulatory role of aspirin

  • Platelets and Thrombosis
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Abstract

Platelet Activating Factor (PAF)-induced human platelet aggregation in citrated plasma is accompanied by activation of the cyclo-oxygenase pathway and release of intracellular constituents including Adenosine-5′-diphosphate (ADP). Inhibition of the cyclo-oxygenase pathway by aspirin prevented the amplification of primary platelet aggregation induced by threshold concentrations of PAF. Removal of ADP by enzymatic systems had little or no effect on PAF-induced full aggregation, but reversed the aggregating effect of PAF (at 10 times threshold concentrations) on ‘aspirinated’ platelets. Aspirin also prevented the synergism between PAF and ADP when subthreshold concentrations of both compounds were combined. Similar results were obtained in heparinized platelet-rich plasma. Thus, ADP may amplify the primary response to PAF but its role is modulated by the availability of the cyclo-oxygenase pathway products.

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  1. Laboratory of Cardiovascular Clinical Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea, 62-20157, Milano, Italy

    Davide Lauri, Chiara Cerletti & Giovanni de Gaetano

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  1. Davide Lauri
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  2. Chiara Cerletti
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  3. Giovanni de Gaetano
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Lauri, D., Cerletti, C. & de Gaetano, G. Contribution of ADP to the amplification of primary platelet aggregation by platelet activating factor (PAF): modulatory role of aspirin. Agents and Actions 17, 506–511 (1986). https://doi.org/10.1007/BF01965522

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  • DOI: https://doi.org/10.1007/BF01965522

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