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The effects of long lasting intravenous infusion of caerulein and some caerulein-like polypeptides on histidine decarboxylase activity of the gastric mucosa of anaesthetized rats

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Abstract

Histidine decarboxylase activity was increased by caerulein, pancreozymin, pentagastrin and six caerulein analogues in doses which elicited acid secretion, whereas doses inactive on secretion did not alter enzyme activity.

The threshold dose of caerulein by intravenous infusion was 1.4 μg/kg per hour. The maxium rate of histamine formation (tenfold control values) was obtained after a 3-hour infusion of 10 μg/kg per hour. With all the doses tested the induction of histidine decarboxylase activity reached a steady state after 3 hours of infusion and persisted as long as the infusion was continued, up to a maximum of 8 hours.

The activity ratio of caerulein to pancreozymin was 3–4 on a molar basis. Caerulein was found to be 200 times more active than pentagastrin. Cycloheximide (10 mg/kg) decreased the effects of caerulein on gastric acid secretion by 70–80% and blocked the induction of the enzyme activity.

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Authors and Affiliations

  1. Institute of Medical Pharmacology, University of Rome, Italy

    P. Melchiorri & N. Sopranzi

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  1. P. Melchiorri
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  2. N. Sopranzi
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Melchiorri, P., Sopranzi, N. The effects of long lasting intravenous infusion of caerulein and some caerulein-like polypeptides on histidine decarboxylase activity of the gastric mucosa of anaesthetized rats. Agents and Actions 2, 58–64 (1971). https://doi.org/10.1007/BF01965383

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  • DOI: https://doi.org/10.1007/BF01965383

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