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A new method for the large scale preparation of antitoxic antibodies exhibiting high specific protective activities

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Summary

A method using polyethylene-glycol and immobilized pepsin for purifying heterologous antitoxic antibodies is described. Using horse antitetanus plasma or sera, F(ab′)2 fragments exhibiting specific activities in the range of 150 IU per mg protein were repeatedly isolated with a yield around 80%. The procedure was scaled up from 200 ml up to 201.

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Authors and Affiliations

  1. Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28, rue du Dr. Roux, F-75724, Paris Cedex 15, France

    B. Favreau, D. Giurgiu & B. Bizzini

Authors
  1. B. Favreau
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  2. D. Giurgiu
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  3. B. Bizzini
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Additional information

Acknowledgment. This work was supported in part by the Institut National de la Santé et de la Recherche Médicale. Contrat de Recherche Libre No. 813 003.

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Favreau, B., Giurgiu, D. & Bizzini, B. A new method for the large scale preparation of antitoxic antibodies exhibiting high specific protective activities. Experientia 39, 483–487 (1983). https://doi.org/10.1007/BF01965165

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