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Monolayer cultures of perikarya isolated from postnatal rat cerebellum

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Summary

Viable cerebellar perikarya of mixed cell type obtained from 7–9-day-old rats were maintained in monolayer culture for up to 12 days. During this time extensive neurite formation and outgrowth occurred. The large majority of the cells developing in culture were tentatively identified as granule neurons. This identification was based on the large number isolated from the starting tissue, and the cells’ general morphological features in culture such as perikaryal and nuclear size, the bipolar nature of neurite extension, and their migratory behaviour.

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References

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Author notes

  1. J. Cohen

    Present address: Medical Research Council Neuroimmunology Project, Department of Zoology, University College London, UK

Authors and Affiliations

  1. Brain Research Group, Department of Biology, The Open University, MK7 6AA, Milton Keynes, England

    D. N. Currie, G. R. Dutton & J. Cohen

Authors
  1. D. N. Currie
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  2. G. R. Dutton
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  3. J. Cohen
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Additional information

D.N. Currie was supported by a grant from Hoechst Pharmaceuticals, Ld, U.K.

J.C. was supported by an S.R.C. Grant to S.P.R. Rose

The authors would like to thank Kathleen Tear for excellent technical assistance and members of the Brain Research Group for helpful discussions.

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Currie, D.N., Dutton, G.R. & Cohen, J. Monolayer cultures of perikarya isolated from postnatal rat cerebellum. Experientia 35, 345–347 (1979). https://doi.org/10.1007/BF01964343

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