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Photosynthesis by isolated chloroplasts ofSorghum vulgare

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Summary

The ambiguous location of photosynthetic carboxylases of mesophyll and bundle sheath chloroplasts of sorghum was investigated after successful homogeneous preparation. The phosphoenol pyruvate carboxylase was found as a particulate enzyme in the mesophyll cell chloroplasts and ribulose 1,5-biphosphate carboxylase in the stroma of the bundle sheath cell chloroplasts. Extensive characterization was carried out on these 2 enzymes for better understanding of the enzyme action.

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Abbreviations

BSA:

bovine serum albumin

C3 :

reductivepentose phosphate cycle

C4 :

C4 dicarboxylic acid cycle

DTT:

dithiothreitol

PEG:

polyethylene glycol

Tris:

tris hydroxymethyl amino-methyl

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Authors and Affiliations

  1. Department of Botany, University of Madras, Autonomous Postgraduate Centre, 641 041, Coimbatore, India

    K. Francis

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  1. K. Francis
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Francis, K. Photosynthesis by isolated chloroplasts ofSorghum vulgare. Experientia 35, 1324–1326 (1979). https://doi.org/10.1007/BF01963986

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