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Heterogeneity of DNA methylation in murine L5178Y lymphoblasts

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Summary

Conparison of the extent of methylation in mouse DNA fragments rendered MgCl2 soluble after mild DNase II digestion of nuclei with different reassociation rate and nucleoti-bound, revealed the existence of 3 regions of the genome particularly 5-methylcytosine-rich: the sequences considered to be related to the transcriptionally active chromatin with the highest content of this base and fast reassociating, as well as nucleolar DNA with sonewhat lower proportion of the methylated cytosines.

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References

  1. G.J. Kirianov, G.A. Romanov and B.F. Vanyushin, Dokl. Akad. Nauk SSSR219, 1007 (1974)

    CAS  PubMed  Google Scholar 

  2. R.L.P. Adams, E.L. McKay, J.T. Douglas and R.H. Burdon, Nucl. Acid Res.4, 3097 (1977).

    Article  CAS  Google Scholar 

  3. M.J. Browne, A.C.B. Cato and R.H. Burdon, FEBS Letters91, 69 (1978).

    Article  CAS  PubMed  Google Scholar 

  4. R.L.P. Adams, J. Thurbull, E. Smillie and R.H. Burdon, in: Postsynthetic modifications of macromolecules, p. 39, Akademiai Kaido, Budapest 1975.

    Google Scholar 

  5. J. Malec, L. Kornacka and M. Wojnarowska, Arch. Immunol. Ther. Exp.23, 693 (1975).

    CAS  Google Scholar 

  6. J.M. Gottesfeld, G. Bagi, B. Berg and J. Bonner, Biochemistry15, 2472 (1976).

    Article  CAS  PubMed  Google Scholar 

  7. J.M. Gotttesfeld and P.J.G. Butler, Nucl. Acid. Res.4, 3155 (1977).

    Article  Google Scholar 

  8. P.H.W. Butterworth, in: Subnuclear Components, p. 295. Ed. G.D. Birnie. Butterworths, London 1976.

    Chapter  Google Scholar 

  9. R.B. Church, in: Molecular Techniques and approaches in Developmental Biology, p. 223. Ed. M.J. Chrispeels, J. Wiley, New York 1973.

    Google Scholar 

  10. M. Muramatsu, K. Smetana and H. Busch, Cancer Res.23, 510 (1963).

    CAS  Google Scholar 

  11. J. Malec, M. Wojanorowska and L. Kornacka, Haematologia5, 25 (1971).

    CAS  PubMed  Google Scholar 

  12. G.R. Wyatt, Biochem. J.48, 584 (1951).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. C.L. Schildkraut and J.J. Maio, Biochim. biophys. Acta161, 76 (1968).

    Article  CAS  PubMed  Google Scholar 

  14. E.H. Davidson and R.J. Britten, Q. Rev. Biol.48, 565 (1973).

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Heamatology, Chocimska 5, PL-00-957, Warsaw, Poland

    J. Sawecka, L. Kormacka & J. Malec

Authors
  1. J. Sawecka
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  2. L. Kormacka
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  3. J. Malec
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Additional information

Acknowledgments. This research was supported by the Polish National Cancer Program PR-6 grant No. 1317/13.

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Sawecka, J., Kormacka, L. & Malec, J. Heterogeneity of DNA methylation in murine L5178Y lymphoblasts. Experientia 35, 1166–1167 (1979). https://doi.org/10.1007/BF01963264

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